Morar Iulia Ioana, Pop Raluca Maria, Peitzner Erik, Ranga Floricuța, Orăsan Meda Sandra, Cecan Andra Diana, Chera Elisabeta Ioana, Bonci Teodora Irina, Usatiuc Lia Oxana, Țicolea Mădălina, But Anca Elena, Cătoi Florinela Adriana, Pârvu Alina Elena, Ghergie Mircea Constantin Dinu
Pathophysiology, Department of Morpho-Functional Sciences, Faculty of Medicine, University of Medicine and Pharmacy "Iuliu Hațieganu", 400012 Cluj-Napoca, Romania.
Pharmacology, Toxicology and Clinical Pharmacology, Department of Morpho-Functional Sciences, Faculty of Medicine, "Iuliu Hațieganu" University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania.
Nutrients. 2025 Jan 13;17(2):276. doi: 10.3390/nu17020276.
Honey is abundant in bioactive compounds, which demonstrate considerable therapeutic effects, particularly on oxidative stress and inflammation.
This work sought to evaluate the antioxidant mechanisms of Manuka honey (MH) and Ohia Lehua honey (OLH), correlating them with phytochemical analyses in a rat model of experimentally induced inflammation.
The identification of polyphenolic compounds in the extracts was carried out using HPLC-ESI MS. The extracts' antioxidant activity was evaluated in vitro through DPPH, FRAP, HO, and NO scavenging assays, while in vivo assessments included measurements of total oxidative status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), advanced oxidation protein products (AOPP), malondialdehyde (MDA), nitric oxide (NO), and total thiols (SH).
The phytochemical analysis found a rich content of phenolic compounds in MH and lower quantities in OLH. In terms of in vitro activity, both MH and OLH exhibited strong DPPH radical scavenging abilities, effective NO and HO scavenging capacities, and high FRAP-reducing power. In vivo, OLH proved highly effective in enhancing antioxidant capacity and lowering oxidative stress markers, showing significant increases in TAC and substantial reductions in TOS and OSI levels. Conversely, MH displayed limited and dose-dependent antioxidant activity, a considerable increase in TAC and SH, and a moderate decrease in TOS and OSI levels.
To our knowledge, this is the first study to assess the phenolic content of OLH and to show its capacity to scavenge free radicals and reduce oxidative stress. The effectiveness of MH primarily relies on its increased antioxidant properties and depends on concentration. These results highlight the importance of investigating natural products in developing antioxidant strategies.
蜂蜜富含生物活性化合物，这些化合物具有显著的治疗作用，尤其是对氧化应激和炎症。
本研究旨在评估麦卢卡蜂蜜（MH）和奥希亚·莱华蜂蜜（OLH）的抗氧化机制，并将其与实验性诱导炎症大鼠模型中的植物化学分析相关联。
使用HPLC-ESI MS对提取物中的多酚化合物进行鉴定。通过DPPH、FRAP、HO和NO清除试验在体外评估提取物的抗氧化活性，而体内评估包括测量总氧化状态（TOS）、总抗氧化能力（TAC）、氧化应激指数（OSI）、晚期氧化蛋白产物（AOPP）、丙二醛（MDA）、一氧化氮（NO）和总硫醇（SH）。
植物化学分析发现MH中酚类化合物含量丰富，而OLH中含量较低。在体外活性方面，MH和OLH均表现出较强的DPPH自由基清除能力、有效的NO和HO清除能力以及较高的FRAP还原能力。在体内，OLH被证明在增强抗氧化能力和降低氧化应激标志物方面非常有效，TAC显著增加，TOS和OSI水平大幅降低。相反，MH表现出有限的剂量依赖性抗氧化活性，TAC和SH显著增加，TOS和OSI水平适度降低。
据我们所知，这是第一项评估OLH酚类含量并显示其清除自由基和降低氧化应激能力的研究。MH的有效性主要依赖于其增加的抗氧化特性，并取决于浓度。这些结果突出了研究天然产物在制定抗氧化策略中的重要性。
