Yao Longmei, Zhi Jiajia, Wang Wenchang, Li Qingyi, Jiang Ding, Chen Xiaohui, Chen Zhidong
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China; Electrolytic Copper Foil Engineering Technology Center of Changzhou University, Changzhou 213164, Jiangsu, China.
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China; Analysis and Testing Center, NERC Biomass of Changzhou University, Jiangsu 213032, China; Electrolytic Copper Foil Engineering Technology Center of Changzhou University, Changzhou 213164, Jiangsu, China.
Bioelectrochemistry. 2025 Oct;165:108987. doi: 10.1016/j.bioelechem.2025.108987. Epub 2025 Apr 8.
As a synthetic estrogen, diethylstilbestrol (DES) residues in water pose a threat to human health. In this work, a novel electrochemiluminescence resonance energy transfer (ECL-RET) aptamer sensor based on NCDs@AgPO as a resonance energy transfer donor and Cu doped Eu MOF (Cu: Eu MOF) as an efficient resonance energy transfer acceptor for the detection of diethylstilbestrol (DES) was proposed for the first time. The aptamer sensor uses AgPO nanoparticles loaded with nitrogen-doped carbon quantum dots (NCDs) as the ECL emitter, which improves the electron transfer efficiency of the sensor and promotes the generation of SO radicals, thereby improving the luminescence intensity and stability of the ECL sensor. The energy acceptor Cu: Eu MOF binds to the apt on NCDs@AgPO by complementary base pairing through complementary DNA (cDNA). When DES is present in the sample, DES competes with the energy acceptor. The stronger interaction between DES and apt leads to the shedding of Cu: Eu MOF-cDNA from the electrode and the recovery of ECL signal. Based on this, under optimal conditions, the linear range of the sensor for detecting DES is 1.0 × 10-1.0 × 10 M, and the detection limit is 7.4 × 10 M (S/N = 3). The developed aptasensor showed excellent recognition ability for residual DES in actual water samples. The sensor has superior measurement ability and potential application value in the field of environmental water quality monitoring.
作为一种合成雌激素，水中的己烯雌酚（DES）残留对人类健康构成威胁。在这项工作中，首次提出了一种基于NCDs@AgPO作为共振能量转移供体和铜掺杂铕金属有机框架（Cu: Eu MOF）作为高效共振能量转移受体的新型电化学发光共振能量转移（ECL-RET）适体传感器，用于检测己烯雌酚（DES）。该适体传感器使用负载氮掺杂碳量子点（NCDs）的AgPO纳米颗粒作为ECL发射体，提高了传感器的电子转移效率，促进了SO自由基的产生，从而提高了ECL传感器的发光强度和稳定性。能量受体Cu: Eu MOF通过互补DNA（cDNA）的互补碱基配对与NCDs@AgPO上的适体结合。当样品中存在DES时，DES与能量受体竞争。DES与适体之间更强的相互作用导致Cu: Eu MOF-cDNA从电极上脱落，ECL信号恢复。基于此，在最佳条件下，该传感器检测DES的线性范围为1.0×10⁻¹.0×10⁻⁶ M，检测限为7.4×10⁻⁷ M（S/N = 3）。所开发的适体传感器对实际水样中的DES残留表现出优异的识别能力。该传感器在环境水质监测领域具有卓越的测量能力和潜在的应用价值。
