Li Zhuanzhuan, Liu Bo, Chen Xi, Ren Jianfeng, Ma Peizhen, Liu Zhihong, Sun Xiujun, Zhou Liqing, Wu Biao, Zheng Yanxin, Yu Tao
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, 266071, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, Shandong, 266237, China.
Sci Data. 2025 Apr 14;12(1):617. doi: 10.1038/s41597-025-04978-x.
The pen shell Atrina pectinata is a bivalve recognized for its outstanding large adductor muscle and developed byssus. Now, it becomes threatened in East Asia, requiring special attention for artificial breeding to boost yield. However, the lack of high-quality genomes hinders our understanding of its reproductive biology, which resulting in the artificial breeding in pen shell is still a scientific technological problem. Here, we produced a high-quality chromosome-level genome assembly of A. pectinata combing the PacBio, Illumina, and high-resolution chromosome conformation capture sequencing. The final assembly has a size of 951.01 Mb with a scaffold N50 of 52.64 Mb, 98.87% of sequence was anchored onto 17 chromosomes, with a BUSCO evaluation integrity score of 98.8%. We successfully identified 29,326 protein-coding genes and 24,708 genes (84.25%) were functionally annotated. The BUSCO evaluation integrity score for the predicted protein-coding genes was 97.7%. This work promotes the applicability of the A. pectinata genome, laying a solid foundation for future investigations into genomics and biology within this species.
栉江珧是一种双壳贝类，以其出色的大型闭壳肌和发达的足丝而闻名。如今，它在东亚受到威胁，需要特别关注人工养殖以提高产量。然而，缺乏高质量的基因组阻碍了我们对其生殖生物学的理解，这导致栉江珧的人工养殖仍然是一个科学技术难题。在此，我们结合PacBio、Illumina和高分辨率染色体构象捕获测序技术，构建了高质量的栉江珧染色体水平基因组组装。最终组装的基因组大小为951.01 Mb，支架N50为52.64 Mb，98.87%的序列被锚定到17条染色体上，BUSCO评估完整性得分98.8%。我们成功鉴定出29326个蛋白质编码基因，其中24708个基因（84.25%）得到了功能注释。预测的蛋白质编码基因的BUSCO评估完整性得分为97.7%。这项工作提高了栉江珧基因组的适用性，为该物种未来的基因组学和生物学研究奠定了坚实基础。
