Chandrashekar R, Rao U R, Parab P B, Subrahmanyam D
Southeast Asian J Trop Med Public Health. 1985 Mar;16(1):15-21.
Sheathed and exsheathed microfilariae of Brugia malayi are killed by normal rat cells in the presence of immune serum in vitro. Immune serum heated at 56 degrees C for 1 hour lost this activity which was largely restored by the addition of fresh normal rat serum. EDTA but not EGTA abolished this activity indicating the operation of complement by alternate pathway. Fresh normal rat serum alone promoted cellular adherence without exerting cytotoxicity to the microfilariae. The activity in the immune serum could be removed with Staphylococcus aureus cells containing Protein A or anti-IgG antiserum. The activity could also be absorbed to and eluted from Protein A--sepharose CL-4B suggesting the involvement of IgG. Neutrophils and macrophages participate in the antibody dependent cell-mediated cytotoxicity phenomenon. Eosinophils while adhering to the microfilariae exert cytotoxicity only to the exsheathed parasites.
在体外，马来布鲁线虫的带鞘和脱鞘微丝蚴在免疫血清存在的情况下会被正常大鼠细胞杀死。在56摄氏度加热1小时的免疫血清失去了这种活性，而添加新鲜的正常大鼠血清后，这种活性在很大程度上得以恢复。乙二胺四乙酸（EDTA）而非乙二醇双乙醚二胺四乙酸（EGTA）消除了这种活性，这表明补体通过替代途径发挥作用。单独的新鲜正常大鼠血清促进细胞黏附，但对微丝蚴不产生细胞毒性。免疫血清中的活性可以用含有A蛋白的金黄色葡萄球菌细胞或抗IgG抗血清去除。这种活性也可以被吸附到A蛋白-琼脂糖CL-4B上并从其上洗脱，这表明IgG参与其中。中性粒细胞和巨噬细胞参与抗体依赖性细胞介导的细胞毒性现象。嗜酸性粒细胞虽然黏附于微丝蚴，但仅对脱鞘寄生虫发挥细胞毒性。
