Chandrashekar R, Rao U R, Subrahmanyam D
Tropenmed Parasitol. 1984 Sep;35(3):177-82.
The effect of diethylcarbamazine citrate (DEC) on serum-dependent cellular adherence and cytotoxicity to sheathed and exsheathed microfilariae of Litomosoides carinii and Brugia pahangi in vitro has been investigated. DEC at 5 micrograms/ml promoted antibody-mediated neutrophil adherence and cytotoxicity with sheathed L. carinii microfilariae. With exsheathed parasites, the drug promoted significantly complement-and antibody-mediated cellular adherence and cytotoxicity. In the case of B. pahangi, antibody-dependent cell-mediated cytotoxicity (ADCC) of neutrophils to sheathed and exsheathed microfilariae were similarly stimulated by DEC. Pre-incubation of microfilariae with DEC promoted the cellular adherence and cytotoxicity whereas pre-incubation of cells had no such effect. DEC stimulated the adherence of eosinophils to both sheathed and exsheathed microfilariae of the two species but the adherence resulted in cytotoxic effect only on the L. carinii microfilariae. DEC at higher concentrations (100 or 500 micrograms/ml) inhibits these processes. Data on the role of different serum factors involved in this phenomenon have been presented and discussed.
已研究了枸橼酸乙胺嗪（DEC）对体外培养的犬恶丝虫和彭亨布鲁线虫带鞘及脱鞘微丝蚴的血清依赖性细胞黏附及细胞毒性的影响。5微克/毫升的DEC可促进抗体介导的中性粒细胞对带鞘犬恶丝虫微丝蚴的黏附及细胞毒性。对于脱鞘寄生虫，该药物可显著促进补体和抗体介导的细胞黏附及细胞毒性。对于彭亨布鲁线虫，DEC同样可刺激中性粒细胞对带鞘及脱鞘微丝蚴的抗体依赖性细胞介导的细胞毒性（ADCC）。微丝蚴与DEC预孵育可促进细胞黏附及细胞毒性，而细胞预孵育则无此作用。DEC可刺激嗜酸性粒细胞对这两种线虫带鞘及脱鞘微丝蚴的黏附，但这种黏附仅对犬恶丝虫微丝蚴产生细胞毒性作用。更高浓度（100或500微克/毫升）的DEC可抑制这些过程。已给出并讨论了参与该现象的不同血清因子的作用数据。
