Kodama M, Noguchi T, Maruyama J, Ogata T, Hashimoto K
J Biochem. 1983 Jan;93(1):243-7. doi: 10.1093/oxfordjournals.jbchem.a134159.
A nontoxic high-molecular fraction was separated from the extracts of toxic liver of a puffer, Takifugu poecilonotus, by Sephadex G-50 gel filtration. This fraction became toxic when digested with RNase T2. The toxins were partially purified by activated charcoal treatment, followed by chromatography on Bio-Gel P-2 and Bio-Rex 70, and were analyzed by TLC and electrophoresis. The results showed that most of the toxicity is accounted for by tetrodotoxin, and the remainder by saxitoxin and other unidentified toxins. The corresponding high-molecular fraction separated from nontoxic liver of another puffer, T. rubripes, did not release any toxin on RNase digestion.
通过葡聚糖G - 50凝胶过滤从豹纹东方鲀有毒肝脏的提取物中分离出一种无毒高分子级分。该级分经核糖核酸酶T2消化后变为有毒。毒素经活性炭处理，然后在生物凝胶P - 2和Bio - Rex 70上进行色谱分离进行部分纯化，并通过薄层层析和电泳进行分析。结果表明，大部分毒性由河豚毒素引起，其余由石房蛤毒素和其他未鉴定的毒素引起。从另一种东方鲀（红鳍东方鲀）无毒肝脏中分离出的相应高分子级分在核糖核酸酶消化后未释放任何毒素。
