Song Hang, Xiao Kang, Min Hanyi, Chen Zhengyu, Long Qin
Department of Ophthalmology, Peking Union Medical College Hospital, Beijing 100730, China.
Key Laboratory of Ocular Fundus Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
J Clin Med. 2022 Feb 21;11(4):1130. doi: 10.3390/jcm11041130.
Conjunctival sac microbiome alterations have been reported to be closely associated with many ocular diseases. However, the characteristic of conjunctival sac microbiome in allergic conjunctivitis (AC) was scarcely described. In this study, we aimed to identify the differences of the conjunctival sac microbiome composition in AC patients compared with normal controls (NCs) using high-throughput 16S rDNA sequencing metagenomic analysis. The conjunctival sac microbiome samples from 28 AC patients and 39 NC patients were collected. The V3-V4 region of 16S rRNA gene high-throughput sequencing was performed on the illumina MiSeq platform. Alpha diversity, beta diversity and the relative abundance at the phylum and genus levels were analyzed using QIIME. Alpha diversity demonstrated by Chao1, Observed_species and PD_whole_tree indexes did not show significant difference between the AC and NC groups, while the Shannon index was higher in the AC group. Beta diversity showed divergent microbiome composition in different groups ( < 0.005). The top five abundant phyla were Firmicutes, Proteobacteria, Actinobacteriota, Bacteroidota and Cyanobacteria in both groups. The top five abundant genera were , and in the AC group and , , , , and in the NC group. The Firmicutes/Bacteroidetes (F/B) ratio at the phylum level was similar between groups ( = 0.144). The () ratio at the genus level was higher in the AC group ( = 0.021). The dysbiosis detected in this study might provide further evidence to investigate the mechanism and treatment methods for allergic conjunctivitis.
据报道，结膜囊微生物群的改变与许多眼部疾病密切相关。然而，过敏性结膜炎（AC）中结膜囊微生物群的特征鲜有描述。在本研究中，我们旨在通过高通量16S rDNA测序宏基因组分析，确定AC患者与正常对照（NC）相比结膜囊微生物群组成的差异。收集了28例AC患者和39例NC患者的结膜囊微生物群样本。在Illumina MiSeq平台上对16S rRNA基因的V3-V4区域进行高通量测序。使用QIIME分析α多样性、β多样性以及门和属水平的相对丰度。Chao1、Observed_species和PD_whole_tree指数显示的α多样性在AC组和NC组之间没有显著差异，而AC组的香农指数更高。β多样性显示不同组的微生物群组成存在差异（<0.005）。两组中丰度最高的前五个门是厚壁菌门、变形菌门、放线菌门、拟杆菌门和蓝细菌门。AC组中丰度最高的前五个属是 ， 和 ，NC组中是 ， ， ， ， 和 。门水平上的厚壁菌门/拟杆菌门（F/B）比率在两组之间相似（=0.144）。AC组中属水平上的 （）比率更高（=0.021）。本研究中检测到的生态失调可能为研究过敏性结膜炎的发病机制和治疗方法提供进一步的证据。
