Perez Lester J, Perez-Restrepo Laura S, Ciuoderis Karl, Usuga Jaime, Moreno Isabel, Vargas Vanessa, Arévalo-Arbelaez Angela J, Berg Michael G, Cloherty Gavin A, Hernández-Ortiz Juan Pablo, Osorio Jorge E
Infectious Diseases Research, Abbott Diagnostics, Chicago, IL, United States.
Abbott Pandemic Defense Coalition, Chicago, IL, United States.
Front Microbiol. 2025 Apr 7;16:1548556. doi: 10.3389/fmicb.2025.1548556. eCollection 2025.
Yellow fever virus (YFV) is an arbovirus that causes acute febrile illness (AFI), in tropical areas of South America and Africa. Through a 2020-2023 AFI study in Leticia, Colombia, leveraging metagenomic next-generation sequencing (mNGS), we identified and isolated YFV (LET1450). Phylogenetic analysis showed this strain belongs to South American genotype II (SamII), linked to Peruvian and Bolivian sequences emerging around 1989. Phylodynamic analysis indicates these strains, with a unique genetic makeup, could have reduced vaccine susceptibility, and due to positive Darwinian selection have an enhanced adaptive capacity. Antigenic analysis identified additional immune-evasive traits and this strain's potential for wider Latin American spread. Phylogeographic reconstruction demonstrated the persistence of YFV in Colombia is not due to repeated external introductions, but results from continuous, cryptic internal circulation. This study highlights the crucial role of mNGS in monitoring emerging strains and underscores the need for genomic surveillance of YFV and other arboviral infections.
黄热病病毒（YFV）是一种虫媒病毒，在南美洲和非洲的热带地区引发急性发热性疾病（AFI）。通过2020年至2023年在哥伦比亚莱蒂西亚开展的一项AFI研究，利用宏基因组新一代测序（mNGS）技术，我们鉴定并分离出了YFV（LET1450）。系统发育分析表明，该毒株属于南美基因型II（SamII），与1989年左右出现的秘鲁和玻利维亚序列相关。系统动力学分析表明，这些具有独特基因构成的毒株可能对疫苗的敏感性降低，并且由于正向达尔文选择而具有增强的适应能力。抗原分析确定了其他免疫逃避特性以及该毒株在拉丁美洲更广泛传播的可能性。系统地理学重建表明，YFV在哥伦比亚的持续存在并非由于反复的外部引入，而是持续的隐匿性内部循环所致。本研究强调了mNGS在监测新出现毒株方面的关键作用，并强调了对YFV和其他虫媒病毒感染进行基因组监测的必要性。
