Zhang Xiaofang, Liu Yuzhen, Cao Quan, Yang Zengzeng, Yu Zehang, Li Caidi, Zhang Chunping, Dong Quanmin
Academy of Animal Science and Veterinary Medicine Qinghai University Xining Qinghai China.
Qinghai Provincial Key Laboratory of Adaptive Management on Alpine Grassland Xining Qinghai China.
Ecol Evol. 2025 Apr 23;15(4):e71149. doi: 10.1002/ece3.71149. eCollection 2025 Apr.
The rhizosphere, as the primary site of plant-soil -microbe interactions, plays an important role in plant productivity. The influence of plant species on rhizosphere soil properties and how this influence shapes the productivity of grassland ecosystems remains poorly understood. Therefore, this study analyzed the changes in bacterial community structure and metabolites in the rhizosphere soil of perennial cultivated grassland. The aim was to elucidate the pathways and underlying mechanisms by which soil physicochemical properties, bacterial community structure, and rhizosphere metabolites influence productivity. Taking perennial monocropping grasslands established with three common grass species from the Qinghai -Tibet Plateau as the research objects, a comprehensive study was conducted using replicated field trials. Rhizosphere soil samples were collected from , , and . The results indicated significant differences in productivity, soil physicochemical properties, bacterial diversity, and community structure among the monocropping grasslands. Among them, ANPP (589.17 g·m), root biomass (3601.67 g·m), moisture (18.15%) and (3.60%) of are higher than those of and , while the relative abundance of (0.82%) is lower than that of and . The topological characteristics of soil bacterial networks varied among the different grass species. The abundances of metabolites consisting of phenylalanine, proline, raffinose, maltotriose, uridine, and 2-methylbenzaldehyde differed among different treatments. Pathway analysis highlighted the upregulation of ABC transporters and pyrimidine metabolism pathways in compared to and . Moreover, secreted more uridine, which prevents the recruitment of pathogenic bacteria (such as ) and promotes the recruitment of , thus improving grassland productivity. These findings show that the productivity of perennial monocropping grasslands around Qinghai Lake is the result of the interaction among rhizosphere soil nutrients, metabolites, and microorganisms. From the ecological point of view, is more conducive to the improvement of forage yield and the restoration of degraded grassland.
根际作为植物 - 土壤 - 微生物相互作用的主要场所，对植物生产力起着重要作用。植物物种对根际土壤性质的影响以及这种影响如何塑造草原生态系统的生产力仍知之甚少。因此，本研究分析了多年生人工草地根际土壤中细菌群落结构和代谢产物的变化。目的是阐明土壤理化性质、细菌群落结构和根际代谢产物影响生产力的途径及潜在机制。以青藏高原三种常见草种建立的多年生单作草地为研究对象，通过重复田间试验进行了综合研究。从[具体位置1]、[具体位置2]和[具体位置3]采集根际土壤样本。结果表明，单作草地在生产力、土壤理化性质、细菌多样性和群落结构方面存在显著差异。其中，[草种1]的地上净初级生产力（ANPP，589.17 g·m）、根系生物量（3601.67 g·m）、水分（18.15%）和[某种物质含量]（3.60%）高于[草种2]和[草种3]，而[某种细菌相对丰度]（0.82%）低于[草种2]和[草种3]。不同草种间土壤细菌网络的拓扑特征各异。不同处理中，由苯丙氨酸、脯氨酸、棉子糖、麦芽三糖、尿苷和2 - 甲基苯甲醛组成的代谢产物丰度不同。通路分析表明，与[草种2]和[草种3]相比，[草种1]中ABC转运蛋白和嘧啶代谢途径上调。此外，[草种1]分泌更多尿苷，可阻止病原菌（如[病原菌名称]）定殖并促进[有益菌名称]定殖，从而提高草地生产力。这些发现表明，青海湖周边多年生单作草地的生产力是根际土壤养分、代谢产物和微生物相互作用的结果。从生态学角度看，[草种1]更有利于提高牧草产量和退化草地的恢复。
