泛癌分析揭示了CCT5在人类肿瘤中的免疫学和预后意义。

Pan-cancer analysis reveals immunological and prognostic significance of CCT5 in human tumors.

作者信息

Ahmed Md Zabir, Billah Md Mohtasim, Ferdous Jannatul, Antar Shoriful Islam, Al Mamun Abdullah, Hossain Md Jubayer

机构信息

Big Bioinformatics Lab (BigBio Lab), Center for Health Innovation, Research, Action, and Learning- Bangladesh (CHIRAL Bangladesh), Dhaka, Bangladesh.

Department of Microbiology, Jagannath University, Dhaka, Bangladesh.

出版信息

Sci Rep. 2025 Apr 24;15(1):14405. doi: 10.1038/s41598-025-88339-z.

DOI:10.1038/s41598-025-88339-z

PMID:40274875

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12022336/

Abstract

The chaperonin containing TCP1 subunit 5 (CCT5) is believed to function as a tumor driver. However, a systematic pan-cancer analysis of CCT5 is still lacking. Therefore, this study aimed to identify the potential role of CCT5 in different types of tumors. This study comprehensively investigated the gene expression, proteomic expression, immune infiltration, DNA methylation, genetic alterations, correlation with TMB and MSI, drug sensitivity, enrichment analysis, and prognostic significance of CCT5 in 33 different tumors based on the TIMER2.0, GEPIA2, UALCAN, SMART, cBioPortal, GSCA databases, and TCGAplot R package. The results revealed significant CCT5 overexpression in most tumors and was significantly associated with poor OS and DFS in different tumor types. Reduced promoter and N-shore methylation of CCT5, indicating its potential oncogenic and epigenetic roles. Amplification was the most common type of CCT5 alterations. Immune infiltration analysis revealed a strong correlation between CCT5 and different immune cells. CCT5 exhibited a significant correlation with TMB and MSI in KIRC and STAD. Furthermore, enrichment analysis revealed associations between CCT5 and cell cycle pathway and various cellular functions. These findings suggested that CCT5 might serve as a potential prognostic biomarker and target for immunotherapy in various cancers.

摘要

含TCP1亚基5的伴侣蛋白（CCT5）被认为具有肿瘤驱动作用。然而，目前仍缺乏对CCT5的系统性泛癌分析。因此，本研究旨在确定CCT5在不同类型肿瘤中的潜在作用。本研究基于TIMER2.0、GEPIA2、UALCAN、SMART、cBioPortal、GSCA数据库以及TCGAplot R包，全面调查了CCT5在33种不同肿瘤中的基因表达、蛋白质组表达、免疫浸润、DNA甲基化、基因改变、与肿瘤突变负荷（TMB）和微卫星不稳定性（MSI）的相关性、药物敏感性、富集分析以及预后意义。结果显示，CCT5在大多数肿瘤中显著过表达，且在不同肿瘤类型中与总生存期（OS）和无病生存期（DFS）较差显著相关。CCT5启动子和N端甲基化降低，表明其具有潜在的致癌和表观遗传作用。扩增是CCT5改变最常见的类型。免疫浸润分析显示CCT5与不同免疫细胞之间存在强烈相关性。在肾透明细胞癌（KIRC）和胃腺癌（STAD）中，CCT5与TMB和MSI显著相关。此外，富集分析揭示了CCT5与细胞周期途径及各种细胞功能之间的关联。这些发现表明，CCT5可能是多种癌症潜在的预后生物标志物和免疫治疗靶点。