Oron U, Mandelberg M
Cell Tissue Res. 1985;241(2):459-63. doi: 10.1007/BF00217194.
The sequential cytological events in the myocardium of the rat were followed for 3 weeks after cold injury by light and electron microscopy. The traumatized area was initially filled with leukocytes and undifferentiated mononucleated cells and subsequently mainly with fibroblasts surrounded by collagen fibers. However, in the margins of the necrotic area repair processes of damaged myocardial cells and probably also the appearance of newly formed cells were evident. The ultrastructural features of these cells were characterized by clusters of ribosomes, numerous mitochondria that were dispersed in the cytoplasm and formation of junctional complexes and transverse tubular systems. Fibrillogenesis was also clearly evident in these cardiomyocytes. The myofibrillar material was initially dispersed in the cytoplasm and associated with clusters of ribosomes and thereafter with presumptive Z-bands and intercalated discs. The myofibrils became further organized in the shape and orientation of those of mature cells two to three weeks after injury. It is concluded that following cold injury regeneration in the mammalian myocardium takes place but is limited to the perinecrotic area. The process resembles the sequential cytological events which occur in cardiomyocytes during embryonic and postnatal development of the ventricular myocardium.
在冷损伤后，通过光学显微镜和电子显微镜对大鼠心肌中的一系列细胞学事件进行了为期3周的观察。创伤区域最初充满白细胞和未分化的单核细胞，随后主要是被胶原纤维包围的成纤维细胞。然而，在坏死区域的边缘，受损心肌细胞的修复过程以及可能新形成细胞的出现是明显的。这些细胞的超微结构特征表现为核糖体簇、分散在细胞质中的大量线粒体以及连接复合体和横管系统的形成。在这些心肌细胞中，原纤维形成也明显可见。肌原纤维物质最初分散在细胞质中，与核糖体簇相关，随后与假定的Z带和闰盘相关。损伤后两到三周，肌原纤维进一步按照成熟细胞的形状和方向进行组织化。得出的结论是，哺乳动物心肌在冷损伤后会发生再生，但仅限于坏死周边区域。这个过程类似于心室心肌在胚胎期和出生后发育过程中心肌细胞中发生的一系列细胞学事件。
