Zhang Hongjuan, Shang Yanmeng, Bai Shuning, Fan Meihua, Sui Xiaolong, Meng Huisheng, Hao Xianjun, Wang Xiangying, Liu Yulin, Li Yi, Hong Jianping, Zhang Jie
College of Resources and Environment, Shanxi Agricultural University, Jinzhong 030801, China.
Soil Health Laboratory in Shanxi Province, Shanxi Agricultural University, Taiyuan 030031, China.
Microorganisms. 2025 Mar 21;13(4):699. doi: 10.3390/microorganisms13040699.
The initial variations in soil bacteria at the very beginning of reclamation still remains unclear. This study investigates the impact on bacterial communities of eight different treatments, including uncultivated land, unfertilized cultivation, chemical fertilizer, chemical fertilizer + bacterial fertilizer, manure, manure + bacterial fertilizer, manure + chemical fertilizer, and manure + chemical fertilizer + bacterial fertilizer, during the short-term reclamation of coal-mining soils. The results showed that total nitrogen, available phosphorus, soil organic carbon, microbial biomass carbon, and alkaline phosphatase activity were significantly increased in all fertilization treatments compared to uncultivated land ( < 0.05). All fertilization treatments other than chemical fertilizer harbored significantly higher activities of urease, catalase, and invertase than unfertilized cultivation ( < 0.05). The bacterial communities structures in manure-amended treatments significantly differed in uncultivated land and unfertilized cultivation and were phylotypically shifted from oligotrophic to -dominant copiotrophic traits, accompanied with phenotypic succession of the enriching characteristics of Gram-positive, biofilms formation, and stress tolerance. The co-occurrence network in manure-amended treatments harbored a simple co-occurrence network, indicating more productive soils than in no-manure treatments. Manure amendment, total nitrogen, microbial biomass carbon, invertase, catalase, and soil moisture were the key driving factors. Our study underscores the bacterial initialization characteristics promoted by manure at the very beginning of coal-mining reclamation.
复垦初期土壤细菌的初始变化仍不清楚。本研究调查了在煤矿土壤短期复垦期间，包括未开垦土地、未施肥耕种、化肥、化肥+菌肥、粪肥、粪肥+菌肥、粪肥+化肥以及粪肥+化肥+菌肥这八种不同处理对细菌群落的影响。结果表明，与未开垦土地相比，所有施肥处理中的全氮、有效磷、土壤有机碳、微生物生物量碳和碱性磷酸酶活性均显著增加（P<0.05）。除化肥处理外，所有施肥处理的脲酶、过氧化氢酶和转化酶活性均显著高于未施肥耕种处理（P<0.05）。粪肥改良处理中的细菌群落结构与未开垦土地和未施肥耕种处理有显著差异，并且在系统发育上从贫营养型转变为富营养型共养特征，同时伴有革兰氏阳性菌富集特征、生物膜形成和胁迫耐受性的表型演替。粪肥改良处理中的共现网络具有简单的共现网络，表明土壤比无粪肥处理更具生产力。粪肥改良、全氮、微生物生物量碳、转化酶、过氧化氢酶和土壤湿度是关键驱动因素。我们的研究强调了在煤矿复垦初期粪肥促进的细菌初始化特征。
