Alexandre Andrei da Silva, Casas Luana Lopes, Silva David Ribeiro da, Nunez Cecilia Veronica
Bioprospecting and Biotechnology Laboratory, Technology and Innovation Coordination, National Institute of Amazonian Research, Manaus 69067-375, AM, Brazil.
Graduate Program in Biotechnology and Natural Resources of the Amazon, School of Health Sciences, Amazonas State University (UEA), Manaus 69050-010, AM, Brazil.
Microorganisms. 2025 Mar 31;13(4):792. doi: 10.3390/microorganisms13040792.
Endophytic fungi are valuable sources of bioactive secondary metabolites, with potential applications in pharmaceutical and agricultural fields. This study investigates the metabolic potential of , an endophytic fungus isolated from . To date, no secondary metabolites have been identified from this species, highlighting the novelty of this research and its contribution to understanding the chemical diversity of endophytic fungi. The fungus was cultivated on parboiled rice under static and dark conditions for 28 days, leading to the isolation of the following three compounds: 5,6-dihydro-5,6-epoxymultiplolide A (), cytosporone C (), and uridine (). Structural identification was carried out using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. The results revealed the metabolic versatility of , as demonstrated by its ability to produce structurally diverse substances with biological relevance. Hence, it describes the first isolation of secondary metabolites from the endophytic fungus , marking a significant step in understanding its chemical profile. The identification of a known antifungal compound and a lactone derivative underscores the biosynthetic potential of this endophytic fungus, while the isolation of a nucleoside expands the chemical repertoire of fungal metabolites, suggesting possible roles in cellular metabolism and stress adaptation. These findings highlight the role of endophytic fungi as prolific sources of structurally diverse and potentially bioactive natural products, supporting further exploration of their biotechnological applications.
内生真菌是生物活性次生代谢产物的宝贵来源，在制药和农业领域具有潜在应用价值。本研究调查了从[具体来源]分离出的内生真菌[真菌名称]的代谢潜力。迄今为止，尚未从该物种中鉴定出次生代谢产物，这突出了本研究的新颖性及其对理解内生真菌化学多样性的贡献。该真菌在半熟大米上于静态和黑暗条件下培养28天，从而分离出以下三种化合物：5,6 - 二氢 - 5,6 - 环氧多洛内酯A（[化合物名称A]）、孢菌素C（[化合物名称B]）和尿苷（[化合物名称C]）。使用核磁共振（NMR）光谱和质谱进行结构鉴定。结果揭示了[真菌名称]的代谢多功能性，这体现在其能够产生具有生物学相关性的结构多样的物质。因此，它描述了首次从内生真菌[真菌名称]中分离出次生代谢产物，这标志着在了解其化学特征方面迈出了重要一步。一种已知抗真菌化合物和一种内酯衍生物的鉴定强调了这种内生真菌的生物合成潜力，而一种核苷的分离扩展了真菌代谢产物的化学种类，暗示了其在细胞代谢和应激适应中的可能作用。这些发现突出了内生真菌作为结构多样且具有潜在生物活性的天然产物丰富来源的作用，支持对其生物技术应用的进一步探索。
