Zambare Rutuja, Bhagwat Vaidehi, Singh Shivangni, Guntha Maheswari, Ghormade Vandana, Tupe Santosh G, Shaikh Shamim, Deshpande Mukund V
R&D (Department of Scientific and Industrial Research recognized), Greenvention Biotech Pvt. Ltd., Uruli Kanchan 412202, India.
Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth (Deemed to Be University), Pune 411046, India.
Microorganisms. 2025 Apr 14;13(4):900. doi: 10.3390/microorganisms13040900.
In the field, substantial quantities of insect pathogenic fungal conidia (5 × 10/ha) are usually applied for the control of pests. In this regard, attempts are being made to obtain higher yields of conidia to make the process viable. One of the approaches is to induce microcycle conidia (MC) production. In a solid-state fermentation on rice, the SYB-grown inoculum with more pseudomycelia of enhanced MC production almost 5 times compared to the aerial conidia (AC) within 10 days. A chitosan (CNP) and alginate-chitosan (ACNP) nanocoating of MC increased the overall temperature and UV stability. The % cumulative mortalities of larvae over 10 d were 83 ± 8.0, 90 ± 5.0, 83 ± 5.0, and 90 ± 6 for AC-, MC-, CNP- coated MC and ACNP-coated MC, respectively. Using probit analysis, the LT values were 5.8, 6.0, 7.5, and 6.3 d for AC, MC, CNPs-MC, and ACNPs-MC, respectively. It was observed that chitin deacetylase (CDA) plays a significant role in increasing MC formation. The higher relative proportion of total CDA over chitosanase activity (higher CDA: chitosanase activity ratio) was found to be correlated with the microcycle conidiation.
在田间，通常施用大量昆虫病原真菌分生孢子（5×10⁹/公顷）来防治害虫。在这方面，人们正在努力提高分生孢子产量以使该过程可行。其中一种方法是诱导微循环分生孢子（MC）产生。在以大米为基质的固态发酵中，与气生分生孢子（AC）相比，生长有更多假菌丝体的SYB接种物在10天内使MC产量提高了近5倍。MC的壳聚糖（CNP）和海藻酸盐 - 壳聚糖（ACNP）纳米涂层提高了其整体温度和紫外线稳定性。10天内幼虫的累积死亡率分别为：AC包被的MC为83±8.0%、MC为90±5.0%、CNP包被的MC为83±5.0%、ACNP包被的MC为90±6%。使用概率分析，AC、MC、CNP - MC和ACNP - MC的致死中时间（LT）值分别为5.8天、6.0天、7.5天和6.3天。据观察，几丁质脱乙酰酶（CDA）在增加MC形成中起重要作用。发现总CDA相对于壳聚糖酶活性的较高相对比例（较高的CDA：壳聚糖酶活性比）与微循环分生孢子形成相关。
