Kovács Flórián, Juhos Katalin, Vizvári Zoltán, Odry Péter, Gyalai Ingrid M, Sarcevic Peter, Odry Ákos
Department of Agro-Environmental Studies, Hungarian University of Agriculture and Life Sciences, Villányi Str. 29-43, H-1118 Budapest, Hungary.
Institute of Plant Sciences and Environmental Protection, Faculty of Agriculture, University of Szeged, H-6800 Hódmezővásárhely, Hungary.
Sensors (Basel). 2025 Apr 15;25(8):2486. doi: 10.3390/s25082486.
Nitrogen (N) availability is critical for cucumber ( L.) growth and yield in greenhouse production. In this study, we investigated the effects of different N doses on the bioimpedance spectroscopy (BIS) parameters of cucumber plants (ES.22.17 F1 genotype), focusing on extracellular fluid resistance (R1), intracellular fluid resistance (R2), vacuole fluid resistance (R4), and cell membrane capacitances (Cm, Ct). The results showed that low N supply significantly increased R1 and reduced Cm in the leaves, indicative of decreased nitrate (NO) concentration and impaired membrane fluidity. Higher N supply lowered resistance and increased cell membrane capacitance, reflecting improved ion transport and storage efficiency. A strong positive correlation was observed between total N and NO content (r = 0.9), while NO content negatively correlated with extracellular fluid resistance (R1, r = -0.8) and vacuole fluid resistance (R4, r = -0.9). The optimal N supply for cucumber plants was associated with R1 values of 47,121.07-52,953.93 Ω, R4 values of 0.348-0.529 Ω, and Cm values of 3.149 × 10⁻-3.781 × 10⁻ F. These BIS parameters showed high sensitivity to plant N status, highlighting BIS as a promising, minimally invasive technique for real-time nutrient monitoring. By integrating BIS data and horticultural best practices, growers can refine N fertilization strategies for better resource efficiency and potentially higher yields and fruit quality.
氮素供应对于温室栽培黄瓜（黄瓜属）的生长和产量至关重要。在本研究中，我们调查了不同氮素剂量对黄瓜植株（ES.22.17 F1基因型）生物电阻抗光谱（BIS）参数的影响，重点关注细胞外液电阻（R1）、细胞内液电阻（R2）、液泡液电阻（R4）和细胞膜电容（Cm、Ct）。结果表明，低氮供应显著增加了叶片中的R1并降低了Cm，这表明硝酸盐（NO）浓度降低且膜流动性受损。较高的氮供应降低了电阻并增加了细胞膜电容，反映出离子运输和储存效率提高。总氮与NO含量之间存在强正相关（r = 0.9），而NO含量与细胞外液电阻（R1，r = -0.8）和液泡液电阻（R4，r = -0.9）呈负相关。黄瓜植株的最佳氮供应与R1值为47,121.07 - 52,953.93 Ω、R4值为0.348 - 0.529 Ω以及Cm值为3.149×10⁻ - 3.781×10⁻ F相关。这些BIS参数对植物氮素状况表现出高敏感性，突出了BIS作为一种有前景的、微创的实时养分监测技术。通过整合BIS数据和园艺最佳实践，种植者可以优化氮肥施用策略，以提高资源利用效率，并可能提高产量和果实品质。
