Department of Environmental Engineering, Faculty of Engineering and Information Technology, University of Pecs, Boszorkany str. 2, Pecs, 7624, Hungary.
Multidisciplinary Medical and Engineering Cellular Bioimpedance Research Group, Szentagothai Research Centre, University of Pecs, Ifjusag str. 20, Pecs, 7624, Hungary.
Sci Rep. 2024 Jul 16;14(1):16380. doi: 10.1038/s41598-024-67061-2.
Bioimpedance spectrum (BIS) measurements have a great future in in vitro experiments, meeting all the requirements for non-destructive and label-free methods. Nevertheless, a real basic research can provide the necessary milestones to achieve the success of the method. In this paper a self-developed technology-based approach for in vitro assays is proposed. Authors invented a special graphene-based measuring plate in order to assess the high sensitivity and reproducibility of introduced technique. The design of the self-produced BIS plates maximizes the detection capacity of qualitative changes in cell culture and it is robust against physical effects and artifacts. The plates do not influence the viability and proliferation, however the results are robust, stable and reproducible regardless of when and where the experiments are carried out. In this study, physiological saline concentrations, two cancer and stem cell lines were utilized. All the results were statistically tested and confirmed. The findings of the assays show, that the introduced BIS technology is appropriate to be used in vitro experiments with high efficacy. The experimental results demonstrate high correlation values across the replicates, and the model parameters suggested that the characteristic differences among the various cell lines can be detected using appropriate hypothesis tests.
生物阻抗谱（BIS）测量在体外实验中有广阔的前景，满足了非破坏性和无标记方法的所有要求。然而，真正的基础研究可以为该方法的成功提供必要的里程碑。本文提出了一种基于自主研发技术的体外检测方法。作者发明了一种特殊的基于石墨烯的测量板，以评估所引入技术的高灵敏度和可重复性。自生产的 BIS 板的设计最大限度地提高了细胞培养中定性变化的检测能力，并且对物理效应和伪影具有很强的鲁棒性。该板不影响细胞活力和增殖，但是无论何时何地进行实验，结果都是稳健、稳定且可重复的。在这项研究中，使用了生理盐水浓度、两种癌细胞和干细胞系。所有结果均经过统计学测试和验证。检测结果表明，所引入的 BIS 技术非常适用于高效的体外实验。实验结果表明，各重复之间的相关值很高，模型参数表明，使用适当的假设检验可以检测到不同细胞系之间的特征差异。
