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脂肪组织来源干细胞的序贯应用可促进复杂伤口愈合：实验模型

Sequential Applications of Adipose Tissue-derived Stem Cells Enhance Healing in Complex Wounds: Experimental Model.

作者信息

Pagotto Vitor P F, Cacere Paula V, Altran Silvana C, Dantas Viviane A N C, Furuya Tatiane K, Santos Deborah L S, Alves Maria José F, Uno Miyuki, Camargo Cristina P, Gemperli Rolf

机构信息

From the Microsurgery and Plastic Surgery Laboratory (LIM04), Faculdade de Medicina, Universidade de São Paulo, São Paulo, São Paulo, Brazil.

Multidisciplinary Research Center, School of Arts, Sciences and Humanities, Universidade de São Paulo, São Paulo, São Paulo, Brazil.

出版信息

Plast Reconstr Surg Glob Open. 2025 Apr 25;13(4):e6718. doi: 10.1097/GOX.0000000000006718. eCollection 2025 Apr.

DOI:10.1097/GOX.0000000000006718
PMID:40291637
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12026421/
Abstract

BACKGROUND

Complex wounds represent a challenge to global health, incurring significant healthcare costs and compromising patients' quality of life. Recently, research has investigated the potential of adipose tissue-derived stem cells (ASC) for therapeutic stem cells' immunomodulatory properties. This study aimed to evaluate the effect of sequential applications of ASC in an experimental model of a complex wound.

METHODS

Thirty male Wistar rats were subjected to a complex wound model of enterocutaneous fistula. After 4 weeks, the rats were divided into 3 groups: control, 2 applications of culture medium, and 2 applications of 1 × 10 allogeneic ASC. The animals were euthanized and analyzed 4 weeks after the first application regarding the macroscopic reduction of the wound, histopathologic changes, and gene expression related to wound healing (, , , , , and ).

RESULTS

Animals treated with ASC showed a reduction in wound diameter of 68% compared with the control group ( = 0.002) and a reduction of 65% compared with the culture medium group ( = 0.011). The ASC group also showed a more than 100% increase in blood vessel count compared with the control group ( = 0.02). Gene expression analysis showed a decrease in the levels in the ASC group compared with the control group.

CONCLUSIONS

This study demonstrated that the treatment with ASC improves the healing of enterocutaneous fistula wounds.

摘要

背景

复杂伤口对全球健康构成挑战，产生高昂的医疗费用并影响患者生活质量。最近，研究调查了脂肪组织来源的干细胞（ASC）作为治疗性干细胞免疫调节特性的潜力。本研究旨在评估在复杂伤口实验模型中序贯应用ASC的效果。

方法

30只雄性Wistar大鼠建立肠皮肤瘘复杂伤口模型。4周后，将大鼠分为3组：对照组、2次应用培养基组和2次应用1×10异体ASC组。首次应用后4周对动物实施安乐死，并分析伤口宏观缩小情况、组织病理学变化以及与伤口愈合相关的基因表达（、、、、和）。

结果

与对照组相比，接受ASC治疗的动物伤口直径缩小了68%（P = 0.002），与培养基组相比缩小了65%（P = 0.011）。与对照组相比，ASC组血管计数增加了100%以上（P = 0.02）。基因表达分析显示，与对照组相比，ASC组水平降低。

结论

本研究表明，ASC治疗可改善肠皮肤瘘伤口的愈合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/1e2f13a9ff41/gox-13-e6718-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/e5ead6ac179f/gox-13-e6718-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/eeea5ec0ea7d/gox-13-e6718-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/159e0fd88e97/gox-13-e6718-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/0ff00bc7b0f9/gox-13-e6718-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/1e2f13a9ff41/gox-13-e6718-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/e5ead6ac179f/gox-13-e6718-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/eeea5ec0ea7d/gox-13-e6718-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/159e0fd88e97/gox-13-e6718-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/0ff00bc7b0f9/gox-13-e6718-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f12/12026421/1e2f13a9ff41/gox-13-e6718-g005.jpg

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本文引用的文献

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Acta Cir Bras. 2023 Oct 13;38:e384523. doi: 10.1590/acb384523. eCollection 2023.
2
Adipose-Derived Stem Cell-Enriched Lipotransfer Reverses Skin Sclerosis by Suppressing Dermal Inflammation.脂肪来源的干细胞富集脂肪移植通过抑制真皮炎症逆转皮肤硬化。
Plast Reconstr Surg. 2022 Sep 1;150(3):578-587. doi: 10.1097/PRS.0000000000009435. Epub 2022 Jun 28.
3
Effects of Adipose-Derived Stem Cells and Their Conditioned Medium in a Human Ex Vivo Wound Model.
脂肪来源干细胞及其条件培养基在人体离体创面模型中的作用。
Cells. 2022 Apr 2;11(7):1198. doi: 10.3390/cells11071198.
4
Bacteria and bioburden and healing in complex wounds: A prognostic systematic review.复杂伤口中的细菌、生物负荷与愈合：一项预后的系统评价。
Wound Repair Regen. 2021 May;29(3):466-477. doi: 10.1111/wrr.12898. Epub 2021 Feb 16.
5
Wound healing: cellular mechanisms and pathological outcomes.伤口愈合：细胞机制和病理结果。
Open Biol. 2020 Sep;10(9):200223. doi: 10.1098/rsob.200223. Epub 2020 Sep 30.
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