Markey S P, Colburn R W, Johannessen J N
Biomed Mass Spectrom. 1981 Jul;8(7):301-4. doi: 10.1002/bms.1200080704.
A gas chromatographic mass spectrometric assay for serotonin (5-hydroxytryptamine) has been developed. Serotonin is acylated in homogenized biological media, efficiently extracted into ethyl acetate, and further derivatized with pentafluoropropionic anhydride. Mass spectral characterization of derivatized serotonin using electron and positive or negative chemical ionization indicates that electron ionization mass spectrometry is useful for serotonin assay in most biological fluids and tissues, but that electron capture negative chemical ionization is required for samples containing less than 1-2 ng (cerebrospinal fluid). An assay limit of 10 pg per sample has been established. Examples of applications for the assay of serotonin in human platelets, rat spinal cord, and monkey cerebrospinal fluid are presented.
已开发出一种用于检测血清素（5-羟色胺）的气相色谱-质谱分析法。血清素在匀浆生物介质中被酰化，有效萃取到乙酸乙酯中，再用五氟丙酸酐进一步衍生化。使用电子电离以及正或负化学电离对衍生化血清素进行质谱表征表明，电子电离质谱法对大多数生物体液和组织中的血清素检测有用，但对于含量低于1 - 2纳克的样本（脑脊液），则需要电子捕获负化学电离。已确定每个样本的检测限为10皮克。文中给出了该分析法在人血小板、大鼠脊髓和猴脑脊液中血清素检测的应用实例。
