Handal Nina, Kaspersen Håkon, Mo Solveig Sølverød, Cabanel Nicolas, Jørgensen Silje Bakken, Fortineau Nicolas, Oueslati Saoussen, Naas Thierry, Glaser Philippe, Sunde Marianne
Department of Microbiology and Infection Control, Division for Diagnostics and Technology, Akershus University Hospital, Lørenskog, Norway.
Department of Animal Health, Welfare and Food Safety, Section for Food Safety and Animal Health Research, Norwegian Veterinary Institute, Ås, Norway.
J Antimicrob Chemother. 2025 Apr 29;80(6):1707-15. doi: 10.1093/jac/dkaf130.
Escherichia coli is the primary aetiological agent of urinary tract infections (UTIs), a frequent cause of antibiotic prescription. Over the years, E. coli has become increasingly resistant towards several antimicrobial drugs used to treat UTIs.
To compare the population structures and antimicrobial resistance profiles by molecular characterization of longitudinally collected E. coli from urine samples at two university hospitals in Norway and France.
Each month in 2019, the first 10 E. coli identified in urine cultures of hospitalized and primary healthcare (PHC) patients were collected at both locations. WGS was performed to detect acquired antimicrobial resistance genes (ARGs) and plasmids and to determine phylogenetic relationships between isolates.
A total of 478 isolates were included, revealing a high diversity of STs among the isolates. However, the same 10 STs (ST73, ST131, ST69, ST95, ST12, ST141, ST127, ST10, ST58 and ST404) were dominant in both countries, constituting 55.9% (n = 267) of all isolates. Seven of the 10 prevalent STs belonged to phylogroup B2. The number of ARGs varied from 0 (n = 229) to 17 (n = 2). The mean ARG was higher in the French (2.9) than in the Norwegian cohort (2.1, P < 0.001), as well as in the hospitalized (3.0) compared with the PHC cohort (2.0, P = 0.007).
Despite the differences in patterns of ARGs between the Norwegian and French cohorts, the E. coli population structure was surprisingly highly conserved, suggesting that the distribution of the most common lineages largely depends on factors other than antibiotic use and resistance.
大肠杆菌是尿路感染（UTIs）的主要病原体，也是抗生素处方的常见原因。多年来，大肠杆菌对几种用于治疗UTIs的抗菌药物的耐药性日益增强。
通过对挪威和法国两家大学医院纵向收集的尿液样本中的大肠杆菌进行分子特征分析，比较其种群结构和抗菌药物耐药谱。
2019年每月在两个地点收集住院患者和初级医疗保健（PHC）患者尿液培养物中鉴定出的前10株大肠杆菌。进行全基因组测序（WGS）以检测获得性抗菌药物耐药基因（ARGs）和质粒，并确定分离株之间的系统发育关系。
共纳入478株分离株，显示分离株之间的序列类型（STs）具有高度多样性。然而，相同的10种STs（ST73、ST131、ST69、ST95、ST12、ST141、ST127、ST10、ST58和ST404）在两国均占主导地位，占所有分离株的55.9%（n = 267）。10种流行STs中的7种属于B2系统发育群。ARGs的数量从0（n = 229）到17（n = 2）不等。法国队列中的平均ARG数（2.9）高于挪威队列（2.1，P < 0.001），住院患者中的平均ARG数（3.0）也高于PHC队列（2.0，P = 0.007）。
尽管挪威和法国队列之间的ARGs模式存在差异，但大肠杆菌的种群结构出人意料地高度保守，这表明最常见谱系的分布在很大程度上取决于抗生素使用和耐药性以外的因素。
