Kerek Ádám, Szabó Ábel, Barnácz Franciska, Csirmaz Bence, Kovács László, Jerzsele Ákos
Department of Pharmacology and Toxicology, University of Veterinary Medicine, István utca 2, H-1078 Budapest, Hungary.
National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, István utca 2, H-1078 Budapest, Hungary.
Antibiotics (Basel). 2025 Apr 17;14(4):413. doi: 10.3390/antibiotics14040413.
The global spread of antimicrobial resistance (AMR) remains one of the greatest challenges of our time, necessitating collaboration among professionals in both the animal and public health sectors. One bacterial species that is developing AMR is . It causes serious bacterial infections and continues to cause significant economic losses in the poultry industry. This study aimed to evaluate the antimicrobial susceptibility profiles of commensal strains isolated from large-scale turkey flocks in Hungary using minimum inhibitory concentration (MIC) determination. We complemented our research with polymerase chain reaction (PCR) analysis to detect the major and minor toxin genes that are characteristic of the species and to explore the potential associations between gene presence and antimicrobial resistance profiles. A total of 146 commensal isolates were examined. Sensitivity to penicillin was reduced, with only 44.5% of isolates remaining susceptible, whereas 87.7% of isolates were sensitive to amoxicillin. The PCR results revealed that all isolates carried the major toxin gene, 23.9% harbored the major toxin gene, 15.8% the minor toxin gene, 3.4% the minor toxin gene, and 2.7% the major toxin gene. No statistically significant associations were observed between the presence of toxin genes and the antimicrobial susceptibility profiles of the isolates; the MIC values showed no correlation with the presence of toxin-producing genes. isolates retained susceptibility to beta-lactam antibiotics, which remain the primary choice for treatment. Regular monitoring can aid in establishing temporal trends. Future studies should include larger sample sizes and employ next-generation sequencing to further investigate multidrug-resistant strains.
抗菌药物耐药性（AMR）的全球传播仍然是我们这个时代最大的挑战之一，这需要动物卫生和公共卫生领域的专业人员开展合作。正在产生AMR的一种细菌是 。它会引发严重的细菌感染，并持续给家禽业造成重大经济损失。本研究旨在通过最低抑菌浓度（MIC）测定来评估从匈牙利大规模火鸡群中分离出的共生 菌株的抗菌药物敏感性谱。我们通过聚合酶链反应（PCR）分析对研究进行补充，以检测该菌种特有的主要和次要毒素基因，并探索基因存在与抗菌药物耐药性谱之间的潜在关联。总共检测了146株共生分离株。对青霉素的敏感性降低，只有44.5%的分离株仍保持敏感，而87.7%的分离株对阿莫西林敏感。PCR结果显示，所有分离株都携带 主要毒素基因，23.9%携带 主要毒素基因，15.8%携带 次要毒素基因，3.4%携带 次要毒素基因，2.7%携带 主要毒素基因。在毒素基因的存在与分离株的抗菌药物敏感性谱之间未观察到统计学上的显著关联；MIC值与产毒素基因的存在没有相关性。 分离株对β-内酰胺类抗生素仍保持敏感，β-内酰胺类抗生素仍是治疗的主要选择。定期监测有助于确定时间趋势。未来的研究应纳入更大的样本量，并采用下一代测序技术进一步研究多重耐药菌株。
