Interaction between the probiotic and Typhimurium in Caco-2 cell culture.

BACKGROUND AND OBJECTIVES

probiotics have been recently considered in biotechnological researches, and food additives. The present study was aimed to investigate the effects of probiotics (PY79 and ATCC 6633) and their metabolites on Typhimurium in Caco-2 cells.

MATERIALS AND METHODS

Cytotoxicity of ATCC 6633 crude supernatant (CS) was evaluated by 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Typhimurium invasion assay was performed in the presence of the probiotics. Cell viability, apoptosis, and necrosis were evaluated in presence of Typhimurium, strains, and CS (4%, 8%) using flow cytometry.

RESULTS

Results showed a significant reduction in the invasive ability of Typhimurium to Caco-2 cells by employing probiotics, and CS (p < 0.05). The less invasion was indicated in PY79 and co-cultural group. Furthermore, the cell survival rates, and apoptosis/necrosis were respectively increased and decreased in co-culture groups (p < 0.05).

CONCLUSION

Hence, it seems that strains could be suggested as beneficial candidates to overcome the invasion and cytotoxicity of on the intestinal cells. However, additional models are suggested to validate our results.