Ostovan Raheleh, Pourmontaseri Maryam, Hosseinzadeh Saeid, Shekarforoush Seyed Shahram
Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Iran J Microbiol. 2021 Feb;13(1):91-97. doi: 10.18502/ijm.v13i1.5497.
probiotics have been recently considered in biotechnological researches, and food additives. The present study was aimed to investigate the effects of probiotics (PY79 and ATCC 6633) and their metabolites on Typhimurium in Caco-2 cells.
Cytotoxicity of ATCC 6633 crude supernatant (CS) was evaluated by 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Typhimurium invasion assay was performed in the presence of the probiotics. Cell viability, apoptosis, and necrosis were evaluated in presence of Typhimurium, strains, and CS (4%, 8%) using flow cytometry.
Results showed a significant reduction in the invasive ability of Typhimurium to Caco-2 cells by employing probiotics, and CS (p < 0.05). The less invasion was indicated in PY79 and co-cultural group. Furthermore, the cell survival rates, and apoptosis/necrosis were respectively increased and decreased in co-culture groups (p < 0.05).
Hence, it seems that strains could be suggested as beneficial candidates to overcome the invasion and cytotoxicity of on the intestinal cells. However, additional models are suggested to validate our results.
益生菌最近在生物技术研究和食品添加剂领域受到关注。本研究旨在探讨益生菌（PY79和ATCC 6633）及其代谢产物对Caco-2细胞中鼠伤寒沙门氏菌的影响。
通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法评估ATCC 6633粗提上清液（CS）的细胞毒性。在益生菌存在的情况下进行鼠伤寒沙门氏菌侵袭试验。使用流式细胞术在鼠伤寒沙门氏菌、菌株和CS（4%、8%）存在的情况下评估细胞活力、凋亡和坏死情况。
结果显示，使用益生菌和CS后，鼠伤寒沙门氏菌对Caco-2细胞的侵袭能力显著降低（p < 0.05）。PY79和共培养组的侵袭较少。此外，共培养组的细胞存活率分别增加，凋亡/坏死率降低（p < 0.05）。
因此，似乎这些菌株可被视为克服[此处原文缺失相关细菌名称]对肠道细胞的侵袭和细胞毒性的有益候选者。然而，建议采用更多模型来验证我们的结果。
