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2019 - 2021年新冠疫情期间里斯本地区室内家猫的SARS-CoV-2血清流行率

SARS-CoV-2 Seroprevalence in Indoor House Cats From the Lisbon Area During the COVID-19 Pandemic, 2019-2021.

作者信息

Moutinho Isa, Henriques Mafalda, Cardoso Sara, da Penha Coutinho Teresa, Penha-Gonçalves Carlos, Demengeot Jocelyne, Castanho Miguel, Tavares Luís, Gil Solange, Nunes Telmo, Aires-da-Silva Frederico

机构信息

Center for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.

Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon, Portugal.

出版信息

Transbound Emerg Dis. 2024 Nov 21;2024:1543922. doi: 10.1155/tbed/1543922. eCollection 2024.

DOI:10.1155/tbed/1543922
PMID:40303070
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12020408/
Abstract

The susceptibility of various animal species to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been studied extensively. Cats have garnered significant concern due to their high susceptibility and proximity to humans. This study aimed to evaluate the susceptibility and antibody response in house cats exposed to SARS-CoV-2 when human infection was spreading in the Lisbon area during the 2019-2021 period. A total of 733 serum samples were collected and characterized from cats admitted to the Veterinary Teaching Hospital of the Faculty of Veterinary Medicine at the University of Lisbon (HEV-FMV-ULisboa). All samples were tested by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin M (IgM) and immunoglobulin G (IgG) anti-SARS-CoV-2 whole Spike and receptor-binding domain (RBD) proteins from the Wuhan-Hu-1 isolate and 14.7% (108/733) tested positive, suggesting exposure to the human virus. Surrogate virus neutralization test (sVNT) against the Wuhan-Hu-1 isolate showed that 20.4% of ELISA positive samples (22/108) harbored neutralizing antibodies against the virus. The 22 most promising serum samples were retested using ELISA and sVNT against Alpha, Delta, and Omicron SARS-CoV-2 variants. Notably, these samples exhibited antibodies that were capable of recognizing and neutralizing these variants. Subsequent neutralization assays confirmed that the serum samples effectively inhibited the infection process of Wuhan-Hu-1 D614G, Delta, and Omicron SARS-CoV-2 pseudotyped viruses. Our findings indicate that cats were exposed to SARS-CoV-2 infection during the pandemic period and generated highly effective and broadly neutralizing antibodies against the virus. Although cats have not been demonstrated to significantly contribute to the spread of SARS-CoV-2, their high susceptibility to asymptomatic infection underscores the importance of investment in preventive surveillance measures. In summary, our study reinforces the notion that cats naturally infected with SARS-CoV-2 represent a valuable anthroponotic disease model in house settings and might be a potential source for the development of antibodies against SARS-CoV-2 in tackling future outbreaks with a One Heath perspective.

摘要

对多种动物物种感染严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的易感性已进行了广泛研究。猫因其高度易感性以及与人类的密切接触而备受关注。本研究旨在评估2019 - 2021年期间在里斯本地区人类感染SARS-CoV-2蔓延时，家猫接触该病毒后的易感性和抗体反应。从里斯本大学兽医学院兽医教学医院（HEV - FMV - ULisboa）收治的猫中总共收集并分析了733份血清样本。所有样本均通过酶联免疫吸附测定（ELISA）检测针对来自武汉 - Hu - 1毒株的免疫球蛋白M（IgM）和免疫球蛋白G（IgG）抗SARS-CoV-2全刺突蛋白和受体结合域（RBD）蛋白，14.7%（108/733）检测呈阳性，表明接触过人类病毒。针对武汉 - Hu - 1毒株的替代病毒中和试验（sVNT）显示，20.4%的ELISA阳性样本（22/108）含有针对该病毒的中和抗体。使用ELISA和针对阿尔法、德尔塔和奥密克戎SARS-CoV-2变体的sVNT对22份最有希望的血清样本进行重新检测。值得注意的是，这些样本表现出能够识别和中和这些变体的抗体。随后的中和试验证实，这些血清样本有效抑制了武汉 - Hu - 1 D614G、德尔塔和奥密克戎SARS-CoV-2假型病毒的感染过程。我们的研究结果表明，在大流行期间猫接触了SARS-CoV-2感染，并产生了针对该病毒的高效且广泛中和的抗体。尽管尚未证明猫对SARS-CoV-2的传播有显著贡献，但其对无症状感染的高度易感性凸显了投资于预防性监测措施的重要性。总之，我们的研究强化了这样一种观念，即自然感染SARS-CoV-2的猫在家养环境中是一种有价值的人畜共患病模型，并且从“同一个健康”的角度来看，可能是开发抗SARS-CoV-2抗体以应对未来疫情爆发的潜在来源。

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Immune Responses and Pathogenesis following Experimental SARS-CoV-2 Infection in Domestic Cats.实验性 SARS-CoV-2 感染家猫后的免疫反应和发病机制。
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