Awaida Aya, El Hachem Roula, Issa Aline, Kallasy Mireille, Zogheib Carla, Hage Wajih
Department of Endodontics, Faculty of Dentistry, Saint Joseph University of Beirut, Beirut, Lebanon.
Craniofacial Research Laboratory, Division of Biomaterials, School of Dentistry, Saint Joseph University of Beirut, Beirut, Lebanon.
Biomater Investig Dent. 2025 Apr 11;12:43065. doi: 10.2340/biid.v12.43065. eCollection 2025.
Endodontic infections are biofilm-mediated, demanding effective biofilm eradication from the root canal. Root canal complexities, coupled with bacterial biofilm resistance, pose challenges to thorough disinfection. Irrigation, particularly with sodium hypochlorite, is crucial in endodontics. Activation techniques, like sonic or ultrasonic oscillations, enhance irrigant penetration and biofilm disruption, improving decontamination and treatment outcomes.The aim of the present study was to evaluate the effectiveness of XP Finisher, EndoUltra, Eddy and Irriflex in the reduction of the multispecies endodontic biofilm formed by
A total of 44 single-rooted mandibular premolars were selected and divided into groups for investigation: Group A: Irriflex, Group B: XP Finisher, Group C: Eddy system, and Group D: EndoUltra system. Multispecies biofilms, comprising and , were cultured and inoculated into the pre-treated dentinal canals, which were then incubated for 16 days. Following this, the canals were subjected to the respective irrigation protocols. Bacterial counts were assessed using sterile paper points and culture techniques post-irrigation. Additionally, four non-inoculated root canals were used as negative controls for comparison.
EndoUltra achieved the highest reduction in Total Bacterial Count (TBC) with a median decrease of 75% (interquartile range [IQR]: 70-80%), significantly better than XP Finisher ( = 0.001) and Irriflex ( = 0.001). Eddy led to a reduction in (PA) with a median decrease of 85% (IQR: 80-90%), significantly outperforming Irriflex ( = 0.001) and XP Finisher ( = 0.001). For (EF), EndoUltra had a median reduction of 70% (IQR: 65-75%), significantly better than Eddy ( = 0.01) and Irriflex ( = 0.001), while XP Finisher resulted in a reduction of 60% (IQR: 55-65%). EndoUltra showed the highest reduction in (ProM) with 80% (IQR: 75-85%), significantly better than Irriflex ( = 0.001) and XP Finisher ( = 0.001), with Eddy also better than Irriflex ( = 0.009). EndoUltra reduced (CA) by 65% (IQR: 60-70%), significantly outperforming XP Finisher ( = 0.001) and Eddy ( = 0.001).
Within its limitations, this study identified EndoUltra as highly effective in reducing bacterial counts, indicating its potential utility in disinfecting root canals. These findings underscore the significance of such methods in enhancing treatment outcomes and addressing root canal infections.
牙髓感染是由生物膜介导的，需要从根管中有效清除生物膜。根管的复杂性以及细菌生物膜的抗性对彻底消毒构成了挑战。冲洗，尤其是使用次氯酸钠冲洗，在牙髓病学中至关重要。诸如声波或超声波振荡等激活技术可增强冲洗液的渗透和生物膜破坏，改善去污效果和治疗结果。本研究的目的是评估XP Finisher、EndoUltra、Eddy和Irriflex在减少由……形成的多菌种牙髓生物膜方面的有效性。
总共选择了44颗单根下颌前磨牙并分为几组进行研究：A组：Irriflex；B组：XP Finisher；C组：Eddy系统；D组：EndoUltra系统。培养包含……和……的多菌种生物膜，并将其接种到预处理的牙本质根管中，然后孵育16天。在此之后，对根管进行各自的冲洗方案。冲洗后使用无菌纸尖和培养技术评估细菌计数。此外，使用四个未接种的根管作为阴性对照进行比较。
EndoUltra在总细菌计数（TBC）方面实现了最高的减少，中位数下降75%（四分位间距[IQR]：70 - 80%），显著优于XP Finisher（ = 0.001）和Irriflex（ = 0.001）。Eddy使……（PA）减少，中位数下降85%（IQR：80 - 90%），显著优于Irriflex（ = 0.001）和XP Finisher（ = 0.001）。对于……（EF），EndoUltra的中位数减少为70%（IQR：65 - 75%），显著优于Eddy（ = 0.01）和Irriflex（ = 0.001），而XP Finisher导致减少60%（IQR：55 - 65%）。EndoUltra在……（ProM）方面的减少最高，为80%（IQR：75 - 85%），显著优于Irriflex（ = 憨掸封赶莩非凤石脯将0.001）和XP Finisher（ = 0.001），Eddy也优于Irriflex（ = 0.009）。EndoUltra使……（CA）减少65%（IQR：60 - 70%），显著优于XP Finisher（ = 0.001）和Eddy（ = 0.001）。
在其局限性范围内，本研究确定EndoUltra在减少细菌计数方面非常有效，表明其在根管消毒中的潜在用途。这些发现强调了此类方法在提高治疗效果和解决根管感染方面的重要性。
