Ghoddusi Jamileh, Moushekhian Siavash, Arian Ehsan, Ghiasi Javad, Forghani Maryam
Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran Endod J. 2019 Winter;14(1):63-67. doi: 10.22037/iej.v14i1.22436.
The purpose of this study was to compare the effectiveness of sonic activation and syringe irrigation of 5.25% sodium hypochlorite in removing the ( ) biofilm.
Root canals of 54 extracted human single-rooted central incisors were prepared with ProTaper S1-S2-F1-F2 and Gates Gliden burs size 1, and 2 at the working length. After sterilization, the root canals were contaminated with suspension and randomly assigned to three groups: G1, conventional syringe irrigation; G2, sonic agitation of NaOCl with Endo Activator system; and G3, no subjected to the mentioned irrigation techniques (negative control). Canals were sampled after the disinfection procedure. The colony forming units (CFU) count was evaluated. Samples were also visualized under fluorescent microscope to count viable bacteria. Data were statistically analyzed using the Kruskal-Wallis and one-way ANOVA followed by Tukey's test (<0.05).
There was a significant reduction in the CFU count after both irrigation techniques. There was no significant difference between two techniques (=0.874). Using bacterial viability kit, Endo Activator displayed the least viable bacteria than the other groups (<0.001) and control group showed the greatest one (<0.001).
In this study, the Endo Activator system was more successful in reducing intratubular viable bacteria compared with NaOCl syringe irrigation alone.
本研究的目的是比较超声激活与用5.25%次氯酸钠进行注射器冲洗在去除（ ）生物膜方面的效果。
用ProTaper S1 - S2 - F1 - F2和1号及2号Gates Gliden钻针在工作长度处对54颗拔除的人单根中切牙的根管进行预备。消毒后，将根管用（ ）悬液污染，并随机分为三组：G1组，传统注射器冲洗；G2组，使用Endo Activator系统对次氯酸钠进行超声搅拌；G3组，不进行上述冲洗技术处理（阴性对照）。消毒程序完成后对根管进行取样。评估菌落形成单位（CFU）计数。还在荧光显微镜下观察样本以计数活菌。数据采用Kruskal - Wallis检验和单因素方差分析，随后进行Tukey检验（<0.05）进行统计学分析。
两种冲洗技术后CFU计数均显著降低。两种技术之间无显著差异（=0.874）。使用细菌活力试剂盒，Endo Activator显示的活菌比其他组最少（<0.001），对照组显示的活菌最多（<0.001）。
在本研究中，与单独使用次氯酸钠注射器冲洗相比，Endo Activator系统在减少根管内活菌方面更成功。
