Reale E, Luciano L, Kühn K
Histochemistry. 1985;82(6):513-8. doi: 10.1007/BF00489971.
The glomerular epithelial cells of the rat kidney fixed by vascular perfusion with an aldehyde solution containing either safranine O or alcian blue (and 0.3 M MgCl2) display filaments which are located close to the outer surface of the plasma membrane. These filaments are similar to those revealed by the same methods in the laminae rarae of the glomerular basement membrane. Alcian blue (and MgCl2) further demonstrates the presence of anionic sites inside the endothelial cell pores of the glomerular and peritubular capillaries, on the luminal surface of endothelial cells of large renal vessels and along the basolateral surface of the epithelial cells of the Bowman capsule and of the proximal convoluted tubule.
用含有番红O或阿尔辛蓝（以及0.3M氯化镁）的醛溶液通过血管灌注固定的大鼠肾脏肾小球上皮细胞，显示出位于质膜外表面附近的细丝。这些细丝与在肾小球基底膜透明层中用相同方法显示的细丝相似。阿尔辛蓝（以及氯化镁）进一步证明了在肾小球和肾小管周围毛细血管的内皮细胞孔内、大肾血管内皮细胞的管腔表面以及沿鲍曼囊和近端曲管上皮细胞的基底外侧表面存在阴离子位点。
