College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005377.
Cells of members of the family , phylum , are generally obligate anaerobic rods. Strains D2Q-14 and D2Q-11 were isolated from sediment of the saline lake Manisi in the Xinjiang Uygur Autonomous Region, PR China. In this study, we employed a polyphasic approach and whole genome analysis of the two isolates. Cells of both isolates were Gram-stain-positive rods that were motile by means of flagella and could utilize sulphate, thiosulphate, elemental sulphur and nitrate as electron acceptors. Phylogenetic analyses based on 16S rRNA gene and whole genome sequences indicated that strains D2Q-14 and D2Q-11 constituted a coherent cluster affiliated to the family . In addition, genome analysis revealed that strain D2Q-14harboured one nonribosomal peptide synthetase gene cluster, making up 1.4 % of the entire genome. The genome-based analysis, including average nucleotide identity, average amino acid identity and DNA-DNA hybridization, biochemical, phenotypic and chemotaxonomic characterization, indicated that strains D2Q-14 and D2Q-11 represented two novel species of a novel genus in the family , for which we propose the names gen. nov., sp. nov. and sp. nov., with the type strains D2Q-14 (=KCTC 15986=MCCC 1K04634) and D2Q-11 (=KCTC 15985=MCCC 1K04391), respectively.
成员的细胞 ， 门 ，通常是专性厌氧棒。D2Q-14 和 D2Q-11 株从中国新疆维吾尔自治区玛纳斯盐湖沉积物中分离出来。在本研究中，我们采用多相方法和对两个分离株的全基因组分析。两株菌的细胞均为革兰氏阳性棒状，通过鞭毛运动，能够利用硫酸盐、硫代硫酸盐、元素硫和硝酸盐作为电子受体。基于 16S rRNA 基因和全基因组序列的系统发育分析表明，菌株 D2Q-14 和 D2Q-11 构成一个与家族一致的连贯聚类 。此外，基因组分析表明，菌株 D2Q-14 含有一个非核糖体肽合成酶基因簇，占整个基因组的 1.4%。基于基因组的分析，包括平均核苷酸同一性、平均氨基酸同一性和 DNA-DNA 杂交、生化、表型和化学分类学特征，表明菌株 D2Q-14 和 D2Q-11 代表家族中新属的两个新种，我们建议使用菌株 D2Q-14(=KCTC 15986=MCCC 1K04634)和 D2Q-11(=KCTC 15985=MCCC 1K04391)作为新属、新种和新种的模式株。
