霍乱弧菌569B基因组的有序克隆DNA图谱及遗传标记的定位
Ordered cloned DNA map of the genome of Vibrio cholerae 569B and localization of genetic markers.
作者信息
Chatterjee S, Mondal A K, Begum N A, Roychoudhury S, Das J
机构信息
Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
出版信息
J Bacteriol. 1998 Feb;180(4):901-8. doi: 10.1128/JB.180.4.901-908.1998.
Abstract
By using a low-resolution macrorestriction map as the foundation (R. Majumder et al., J. Bacteriol. 176:1105-1112, 1996), an ordered cloned DNA map of the 3.2-Mb chromosome of the hypertoxinogenic strain 569B of Vibrio cholerae has been constructed. A cosmid library the size of about 4,000 clones containing more than 120 Mb of V. cholerae genomic DNA (40-genome equivalent) was generated. By combining landmark analysis and chromosome walking, the cosmid clones were assembled into 13 contigs covering about 90% of the V. cholerae genome. A total of 92 cosmid clones were assigned to the genome and to regions defined by NotI, SfiI, and CeuI macrorestriction maps. Twenty-seven cloned genes, 9 rrn operons, and 10 copies of a repetitive DNA sequence (IS1004) have been positioned on the ordered cloned DNA map.
摘要
以低分辨率的宏观限制酶切图谱为基础（R. 马宗德等人，《细菌学杂志》176:1105 - 1112，1996年），构建了霍乱弧菌高毒素产生菌株569B 3.2兆碱基染色体的有序克隆DNA图谱。构建了一个约4000个克隆的黏粒文库，其中包含超过120兆碱基的霍乱弧菌基因组DNA（相当于40个基因组）。通过结合地标分析和染色体步移，将黏粒克隆组装成13个重叠群，覆盖了约90%的霍乱弧菌基因组。总共92个黏粒克隆被定位到基因组以及由NotI、SfiI和CeuI宏观限制酶切图谱定义的区域。27个克隆基因、9个rrn操纵子和10个重复DNA序列（IS1004）的拷贝已定位在有序克隆DNA图谱上。
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