Song Xinwei, Wang Yiling, Wang Youjing, Zhao Kankan, Tong Di, Gao Ruichuan, Lv Xiaofei, Kong Dedong, Ruan Yunjie, Wang Mengcen, Tang Xianjin, Li Fangbai, Luo Yongming, Zhu Yongguan, Xu Jianming, Ma Bin
State Key Laboratory of Soil Pollution Control and Safety, Zhejiang University, Hangzhou, 310058, China.
Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 311200, China.
Nat Commun. 2025 Apr 30;16(1):4048. doi: 10.1038/s41467-025-58695-5.
The rhizosphere is a critical hotspot for metabolic activities involving arsenic (As). While recent studies indicate many functions for soil viruses, much remains overlooked regarding their quantitative impact on rhizosphere processes. Here, we analyze time-series metagenomes of rice (Oryza sativa L.)rhizosphere and bulk soil to explore how viruses mediate rhizosphere As biogeochemistry. We observe the rhizosphere favors lysogeny in viruses associated with As-oxidizing microbes, with a positive correlation between As oxidation and the prevalence of these microbial hosts. Moreover, results demonstrate these lysogenic viruses enrich both As oxidation and phosphorus co-metabolism genes and mediated horizontal gene transfers (HGTs) of As oxidases. In silico simulation with genome-scale metabolic models (GEMs) and in vitro validation with experiments estimate that rhizosphere lysogenic viruses contribute up to 25% of microbial As oxidation. These findings enhance our comprehension of the plant-microbiome-virome interplay and highlight the potential of rhizosphere viruses for improving soil health in sustainable agriculture.
根际是涉及砷（As）代谢活动的关键热点区域。虽然最近的研究表明土壤病毒具有多种功能，但它们对根际过程的定量影响仍被大量忽视。在此，我们分析了水稻（Oryza sativa L.）根际和大田土壤的时间序列宏基因组，以探究病毒如何介导根际砷生物地球化学过程。我们观察到根际有利于与砷氧化微生物相关的病毒进行溶原化，砷氧化与这些微生物宿主的丰度之间呈正相关。此外，结果表明这些溶原性病毒富集了砷氧化和磷共代谢基因，并介导了砷氧化酶的水平基因转移（HGTs）。利用基因组规模代谢模型（GEMs）进行的计算机模拟以及实验的体外验证估计，根际溶原性病毒对微生物砷氧化的贡献高达25%。这些发现增强了我们对植物-微生物组-病毒组相互作用的理解，并突出了根际病毒在可持续农业中改善土壤健康的潜力。
