Aili Daniel, Herland Anna
Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Methods Mol Biol. 2025;2924:223-233. doi: 10.1007/978-1-0716-4530-7_16.
This protocol outlines the synthesis and use of engineered hyaluronan-based hydrogels for 3D cell culture and bioprinting of human induced pluripotent stem cell (hiPSC)-derived neuroepithelial stem cells (lt-NES). Key steps include hydrogel formation using bioorthogonal chemistries, cell encapsulation, and 3D bioprinting with a Cellink BioX printer, enabling the creation of complex tissue models. The protocol ensures high cell viability and supports differentiation, essential for neuroscience research and drug development.
本方案概述了基于工程化透明质酸的水凝胶的合成及用于人诱导多能干细胞（hiPSC）来源的神经上皮干细胞（lt-NES）的3D细胞培养和生物打印。关键步骤包括使用生物正交化学方法形成水凝胶、细胞封装以及使用Cellink BioX打印机进行3D生物打印，从而能够创建复杂的组织模型。该方案确保了高细胞活力并支持分化，这对于神经科学研究和药物开发至关重要。
