Barik Binapani, Satapathy Bhabani Sankar, Acharya Biswajeet, Pattnaik Gurudutta, Sillanpää Mika, Al-Farraj Saleh A, Alkahtane Abdullah A
School of Pharmacy and Life Sciences, Centurion University of Technology and Management, Odisha, India.
GITAM School of Pharmacy, GITAM Deemed to be University, Hyderabad Campus, Telangana, 502329, India.
Int J Nanomedicine. 2025 Apr 26;20:5337-5352. doi: 10.2147/IJN.S515396. eCollection 2025.
Silver nanoparticles (AgNPs) have emerged as a promising therapeutic modality in periodontal disease management due to their potent antimicrobial activity and ability to promote tissue regeneration. This study aimed to evaluate the efficacy of AgNP-infused patches in ligature-induced periodontitis rat model.
AgNPs were synthesized using a green synthesis method with neem () leaf extract followed by incorporation into polyvinyl alcohol (PVA)-based patches. In vivo efficacy of experimental AgNP patch was tested in albino rats marketed formulation and control groups, following ARRIVE guidelines.
Characterization by Atomic Force Microscopy revealed spherical AgNPs with an average size of 85 nm, while Field Emission Scanning Electron Microscopy confirmed uniform morphology and size distribution. Production of microporous AgNP patches with uniform nanoparticle dispersion was also validated by Scanning electron microscopy and Energy Dispersive X-ray Analysis. Observational and histopathological analysis revealed significant improvements in gingival and periodontal tissue restoration, inflammation reduction in the AgNP-treated groups compared to untreated and standard-treated groups.
Notably, the high-dose AgNP patch (1000 mg/kg) treated group exhibited near-complete tissue restoration. These findings highlight the potential of AgNP patch as a localized treatment alternative for periodontal disease.
由于银纳米颗粒（AgNPs）具有强大的抗菌活性和促进组织再生的能力，已成为牙周疾病治疗中一种有前景的治疗方式。本研究旨在评估注入AgNP的贴片在结扎诱导的牙周炎大鼠模型中的疗效。
采用绿色合成方法，用印楝叶提取物合成AgNPs，然后将其掺入基于聚乙烯醇（PVA）的贴片中。按照动物研究：体内实验报告指南（ARRIVE），在白化大鼠、市售制剂和对照组中测试实验性AgNP贴片的体内疗效。
原子力显微镜表征显示平均尺寸为85nm的球形AgNPs，场发射扫描电子显微镜证实了其形态和尺寸分布均匀。扫描电子显微镜和能量色散X射线分析也验证了具有均匀纳米颗粒分散的微孔AgNP贴片的生产。观察和组织病理学分析显示，与未治疗和标准治疗组相比，AgNP治疗组的牙龈和牙周组织恢复有显著改善，炎症减轻。
值得注意的是，高剂量AgNP贴片（1000mg/kg）治疗组表现出近乎完全的组织恢复。这些发现突出了AgNP贴片作为牙周疾病局部治疗替代方案的潜力。
