体外动粒的特性。I. 微管成核作用与微管蛋白结合。
Properties of the kinetochore in vitro. I. Microtubule nucleation and tubulin binding.
作者信息
Mitchison T J, Kirschner M W
出版信息
J Cell Biol. 1985 Sep;101(3):755-65. doi: 10.1083/jcb.101.3.755.
Abstract
We have isolated chromosomes from Chinese hamster ovary cells arrested in mitosis with vinblastine and examined the interactions of their kinetochores with purified tubulin in vitro. The kinetochores nucleate microtubule (MT) growth with complex kinetics. After an initial lag phase, MTs are continuously nucleated with both plus and minus ends distally localized. This mixed polarity seems inconsistent with the formation of an ordered, homopolar kinetochore fiber in vivo. As isolated from vinblastine-arrested cells, kinetochores contain no bound tubulin. The kinetochores of chromosomes isolated from colcemid-arrested cells or of chromosomes incubated with tubulin in vitro are brightly stained after anti-tubulin immunofluorescence. This bound tubulin is probably not in the form of MTs. It is localized to the corona region by immunoelectron microscopy, where it may play a role in MT nucleation in vitro.
摘要
我们从用长春花碱阻滞在有丝分裂期的中国仓鼠卵巢细胞中分离出染色体，并在体外研究了它们的动粒与纯化微管蛋白的相互作用。动粒以复杂的动力学方式使微管（MT）生长成核。在最初的延迟期之后，微管持续成核，其正端和负端都位于远端。这种混合极性似乎与体内有序的、同极动粒纤维的形成不一致。从长春花碱阻滞的细胞中分离出的动粒不含有结合的微管蛋白。从秋水仙酰胺阻滞的细胞中分离出的染色体的动粒或在体外与微管蛋白一起孵育的染色体的动粒，在抗微管蛋白免疫荧光后被明亮地染色。这种结合的微管蛋白可能不是微管的形式。通过免疫电子显微镜观察，它定位于冠区，在那里它可能在体外微管成核中起作用。
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本文引用的文献
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Origin of kinetochore microtubules in Chinese hamster ovary cells.中国仓鼠卵巢细胞中动粒微管的起源
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Visualization of the structural polarity of microtubules.微管结构极性的可视化。
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Polarity of microtubules nucleated by centrosomes and chromosomes of Chinese hamster ovary cells in vitro.体外培养的中国仓鼠卵巢细胞中由中心体和染色体成核的微管极性
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Nucleated assembly of mitotic microtubules in living PTK2 cells after release from nocodazole treatment.从诺考达唑处理中释放后，活的PTK2细胞中有丝分裂微管的有核组装。
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The structure of the cold-stable kinetochore fiber in metaphase PtK1 cells.中期PtK1细胞中冷稳定动粒纤维的结构。
Chromosoma. 1981;84(1):145-58. doi: 10.1007/BF00293368.
6
The attachment of kinetochores to the pro-metaphase spindle in PtK1 cells. Recovery from low temperature treatment.PtK1细胞中动粒与前中期纺锤体的附着。低温处理后的恢复。
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7
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