Pailler F M, Patricot M C, Giroud-Argoud M, Revol A
J Endocrinol Invest. 1985 Jun;8(3):265-8. doi: 10.1007/BF03348491.
The authors suggest a method for the evaluation of the urinary 5 alpha-androstane-3 alpha, 17 beta-diol (5 Ad) carried out by gas-liquid chromatography. 5-Ad is separated from its isomers, first by means of an adsorption chromatography on alumina and then by means of a high performance liquid chromatography on porous silica. The reliability criteria of the method have been studied according to the advice of the French Society of Clinical Biology. This method, applied to 175 healthy individuals of both sexes, grouped according to age starting from three years, allowed to determine the usual values of urinary excretion of 5-Ad in relation to age.
作者们提出了一种通过气液色谱法评估尿中5α-雄甾烷-3α,17β-二醇（5-Ad）的方法。5-Ad先通过氧化铝吸附色谱法与其异构体分离，然后通过多孔硅胶高效液相色谱法进一步分离。该方法的可靠性标准已根据法国临床生物学协会的建议进行了研究。此方法应用于175名不同性别的健康个体，这些个体按年龄分组，从3岁开始，从而得以确定尿中5-Ad排泄量与年龄相关的正常数值。
