Sun Yang, Liu Kai, Zhang Haoli, Zhao Yuecan, Wen Jingshuai, Zhao Meng, Li Xiang, Li Zhengping
Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, PR China.
Sci Adv. 2025 May 2;11(18):eadu2271. doi: 10.1126/sciadv.adu2271.
Affordable, sensitive, and simplified DNA/RNA detection is important for disease diagnosis and enables timely medical intervention measures. Usually, high sensitivity depends on expensive instruments and sophisticated procedures, making sensitivity contradict affordability and simplicity. Here, we proposed an ultra-sensitive single-tube biosensor (USTB) where users can visually detect targets by observing the liquid motion state in a glass tube. The developed instrument-free USTB performed low-cost ($0.1), fast (1 min), and ultra-sensitive detection for both the DNA/RNA fragments (≤1 aM) and the clinical positive samples, which commercial reverse transcription polymerase chain reaction (RT-PCR) and PCR kits could not effectively recognize. Furthermore, USTB is promising to be easily applied to detect other-type biomarkers by the designed smart sensing unit.
经济实惠、灵敏且简化的DNA/RNA检测对于疾病诊断至关重要，并能实现及时的医疗干预措施。通常，高灵敏度依赖于昂贵的仪器和复杂的程序，这使得灵敏度与经济实惠性和简易性相互矛盾。在此，我们提出了一种超灵敏单管生物传感器（USTB），用户可以通过观察玻璃管中的液体运动状态直观地检测目标。所开发的无需仪器的USTB对DNA/RNA片段（≤1 aM）和临床阳性样本均能进行低成本（0.1美元）、快速（1分钟）且超灵敏的检测，而商业逆转录聚合酶链反应（RT-PCR）和PCR试剂盒无法有效识别这些样本。此外，通过设计的智能传感单元，USTB有望轻松应用于检测其他类型的生物标志物。
