Bolhari Behnam, Khouzestani Neda Kardouni, Assadian Hadi, Farzad-Mohajeri Saeed, Dehghan Mohammad Mehdi, Niavarzi Soheil, Dorost Behnam, Nagendrababu Venkateshbabu, Duncan Henry F, Heboyan Artak, Signore Antonio, Benedicenti Stefano
Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Sci Rep. 2025 May 7;15(1):15957. doi: 10.1038/s41598-025-00061-y.
The addition of zinc oxide (ZnO) to mineral trioxide aggregate (MTA) has been shown to prevent tooth discoloration; however, its biological effects remain unclear. This study aimed to evaluate the pulpal responses to MTA containing 5% ZnO in full pulpotomy of dogs' teeth. Forty caries-free premolars from mixed-breed dogs were subjected to full pulpotomy. Exposed pulpal tissues were randomly capped with either Angelus MTA (MTA) or Angelus MTA mixed with 5% ZnO (MTA + ZnO) (n = 20 each). After 4 weeks, the teeth were extracted, processed for histological evaluation, and stained with hematoxylin and eosin. Tissue response data were analyzed using the Mann-Whitney U test at a 95% significance level. The incidence, thickness, and continuity of hard-tissue bridge formation were significantly lower in the MTA + ZnO group (p = 0.007, p = 0.001, and p = 0.002, respectively). Most samples in both groups exhibited no inflammatory cells, and none showed signs of necrosis. Incorporating ZnO into Angelus MTA compromised the quantity and quality of hard-tissue bridge formation following full pulpotomy in dogs' premolars.
已证明在矿物三氧化物凝聚体（MTA）中添加氧化锌（ZnO）可防止牙齿变色；然而，其生物学效应仍不清楚。本研究旨在评估犬牙全牙髓切断术中含5%ZnO的MTA对牙髓的反应。从杂种犬中选取40颗无龋前磨牙进行全牙髓切断术。暴露的牙髓组织随机用安杰卢斯MTA（MTA）或与5%ZnO混合的安杰卢斯MTA（MTA + ZnO）覆盖（每组n = 20）。4周后，拔除牙齿，进行组织学评估处理，并用苏木精和伊红染色。使用曼-惠特尼U检验在95%显著性水平下分析组织反应数据。MTA + ZnO组硬组织桥形成的发生率、厚度和连续性显著较低（分别为p = 0.007、p = 0.001和p = 0.002）。两组中的大多数样本均未出现炎性细胞，且均未显示坏死迹象。在犬前磨牙全牙髓切断术后，将ZnO掺入安杰卢斯MTA会损害硬组织桥形成的数量和质量。
