Laboratory of Bio-Physio Sensors and Nanobioengineering, School of Biochemical Engineering, Indian Institute of Technology (BHU) Varanasi, Varanasi, Uttar Pradesh 221005, India.
Laboratory of Bio-Physio Sensors and Nanobioengineering, School of Biochemical Engineering, Indian Institute of Technology (BHU) Varanasi, Varanasi, Uttar Pradesh 221005, India.
Biosens Bioelectron. 2020 Oct 1;165:112361. doi: 10.1016/j.bios.2020.112361. Epub 2020 Jun 8.
The recent outbreak of the coronavirus disease (COVID-19) has left the world clueless. As the WHO declares this new contagion as a pandemic on the 11 of March 2020, the alarming rate of the spawn of the disease in such a short period has disarranged the globe. Standing against this situation researchers are strenuously searching for the key traits responsible for this pandemic. As knowledge regarding the dynamics and host-path interaction of COVID-19 causing Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is currently unknown, the formulation of strategies concerning antiviral treatment, vaccination, and epidemiological control stands crucial. Before designing adequate therapeutic strategies, it is extremely essential to diagnose the disease at the outset as early detection can have a greater impact on building health system capacity. Hence, a comprehensive review of strategies for COVID-19 diagnosis is essential in this existing global situation. In this review, sequentially, we have provided the clinical details along with genetic and proteomic biomarkers related to COVID-19. The article systematically enlightens a clear overview of the clinically adopted techniques for the detection of COVID-19 including oligonucleotide-based molecular detection, Point-of-Care immunodiagnostics, radiographical analysis/sensing system, and newly developed biosensing prototypes having commercial viability. The commercial kits/analytical methods based-sensing strategies have also been tabulated categorically. The critical insights on the developer, commercial brand name, detection methods, technical operational details, detection time, clinical specimen, status, the limit of detection/detection ability have been discussed comprehensively. We believe that this review may provide scientists, clinicians and healthcare manufacturers valuable information regarding the most recent developments/approaches towards COVID-19 diagnosis.
最近爆发的冠状病毒病（COVID-19）使世界感到茫然。随着世界卫生组织于 2020 年 3 月 11 日宣布这种新传染病为大流行，这种疾病在如此短的时间内惊人的繁殖速度扰乱了全球。面对这种情况，研究人员正在努力寻找导致这种大流行的关键特征。由于目前尚不清楚 COVID-19 引起的严重急性呼吸系统综合征冠状病毒-2（SARS-CoV-2）的动力学和宿主-病毒相互作用的知识，因此关于抗病毒治疗、疫苗接种和流行病学控制的策略的制定至关重要。在制定充分的治疗策略之前，从一开始就诊断出这种疾病极为重要，因为早期发现可以对建立卫生系统能力产生更大的影响。因此，在当前的全球形势下，对 COVID-19 诊断策略进行全面审查是必不可少的。在这篇综述中，我们依次提供了与 COVID-19 相关的临床细节以及遗传和蛋白质组生物标志物。本文系统地阐明了用于检测 COVID-19 的临床采用技术的概述，包括基于寡核苷酸的分子检测、即时免疫诊断、放射分析/传感系统以及具有商业可行性的新开发的生物传感原型。还分类列出了基于商业试剂盒/分析方法的传感策略。我们全面讨论了开发商、商业品牌名称、检测方法、技术操作细节、检测时间、临床标本、状态、检测限/检测能力的关键见解。我们相信，这篇综述可能为科学家、临床医生和医疗保健制造商提供有关 COVID-19 诊断的最新发展/方法的有价值的信息。
