Hiratsuka Toru, Rognoni Emanuel
Department of Oncogenesis and Growth Regulation, Research Center, Osaka International Cancer Institute, Chuoku, Osaka 541-8567, Japan; Department of Molcular Oncology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Centre for Cell Biology and Cutaneous Research, The Faculty of Medicine and Dentistry, Blizard Institute, Queen Mary University of London, London, UK.
STAR Protoc. 2025 May 6;6(2):103801. doi: 10.1016/j.xpro.2025.103801.
Dermal fibroblasts play a central role in the production and remodeling of dermal extracellular matrix. Their spatially distinct subpopulations exhibit different functions during skin homeostasis, wound healing, and other diseases such as fibrosis and cancer. Here, we present a protocol for detecting 3D dermal fibroblast cellular dynamics in mice without fixing the tissue using an intravital imaging technique. We describe steps for preparing, labeling, and mounting mice. We then describe detailed procedures for intravital imaging, recovery, and quantification. For complete details on the use and execution of this protocol, please refer to Rognoni et al..
真皮成纤维细胞在真皮细胞外基质的产生和重塑过程中发挥着核心作用。它们在空间上不同的亚群在皮肤稳态、伤口愈合以及诸如纤维化和癌症等其他疾病过程中表现出不同的功能。在此，我们展示了一种使用活体成像技术在不固定组织的情况下检测小鼠三维真皮成纤维细胞细胞动力学的方案。我们描述了制备、标记和固定小鼠的步骤。然后我们详细描述了活体成像、恢复和定量的程序。有关此方案的使用和执行的完整详细信息，请参考罗尼奥尼等人的研究。
