Wanasingha Nisal, Balu Rajkamal, Gangadoo Sheeana, Abraham Amanda N, Rekas Agata, Mata Jitendra P, Le Brun Anton P, Dutta Naba K, Choudhury Namita Roy
Chemical and Environmental Engineering, School of Engineering, RMIT University, Melbourne, VIC 3000, Australia.
ARC Centre of Excellence for Nanoscale BioPhotonics, School of Science, RMIT University, Melbourne, VIC 3000, Australia.
Adv Colloid Interface Sci. 2025 Aug;342:103530. doi: 10.1016/j.cis.2025.103530. Epub 2025 Apr 28.
The applications of responsive biomaterials for tuning cell-surface interactions have been recently explored due to their unique switchable characteristics. However, rational design of surfaces using suitable biomacromolecules to attain optimal physicochemical performance, biocompatibility, cell adhesion and anti-fouling properties is quite challenging. Resilin-mimetic polypeptides (RMPs) are intrinsically disordered biomacromolecules that exhibit multi-stimuli responsive behaviour, including reversible dual-phase thermal behaviour forming self-assembled nano- to microstructures. However, there is a limited understanding of the effect of morphological features of RMP-based nanostructures, and their influence on surface properties. Therefore, in this study, a family of responsive RMP-based nanostructured coatings (nano-coacervates, nanogels and nano-bioconjugates) are fabricated to investigate their various surface properties that influence cell-surface interactions. The effects of their physicochemical properties, such as conformation, packing density, charge, roughness, and stiffness, are investigated using atomic force microscopy, neutron scattering and reflectometry techniques. Biocompatibility and microbiological testing show that these nanostructured switchable responsive coatings can be applied to a wide range of substrates to modulate biofilm formation and attribute antimicrobial characteristics. The developed nanocoatings have the potential to find applications in many areas, including implantable medical devices, and drug delivery.
由于其独特的可切换特性，近年来人们对响应性生物材料在调节细胞-表面相互作用方面的应用进行了探索。然而，利用合适的生物大分子进行表面的合理设计以获得最佳的物理化学性能、生物相容性、细胞粘附和抗污性能颇具挑战性。类弹性蛋白多肽（RMPs）是内在无序的生物大分子，表现出多刺激响应行为，包括形成自组装纳米到微观结构的可逆双相热行为。然而，人们对基于RMP的纳米结构的形态特征及其对表面性质的影响了解有限。因此，在本研究中，制备了一系列基于响应性RMP的纳米结构涂层（纳米凝聚层、纳米凝胶和纳米生物共轭物），以研究它们影响细胞-表面相互作用的各种表面性质。使用原子力显微镜、中子散射和反射测量技术研究了它们的物理化学性质，如构象、堆积密度、电荷、粗糙度和硬度的影响。生物相容性和微生物测试表明，这些纳米结构的可切换响应涂层可应用于多种基材，以调节生物膜形成并赋予抗菌特性。所开发的纳米涂层有潜力在许多领域找到应用，包括可植入医疗设备和药物递送。
