Zdraljevic Stefan, Walter-McNeill Laura, Lee Alex, Bloom Joshua, Kruglyak Leonid
Department of Human Genetics, University of California, Los Angeles, CA, USA.
Department of Biological Chemistry, University of California, Los Angeles, CA, USA.
MicroPubl Biol. 2025 Apr 23;2025. doi: 10.17912/micropub.biology.001544. eCollection 2025.
is a tractable model system that enables the identification of genetic determinants that underlie phenotypic variation. Over the years, new approaches have been developed to lower the cost of and expedite genetic mapping in this model system. The X-QTL approach uses the allele to create obligate outcrossing recombinant populations for selection and sequencing experiments. Here, we tested whether the allele is essential to the X-QTL approach by comparing crosses between the N2 and XZ1516 strains using either or RNAi knockdown to facilitate outcrossing. The genome-wide allele frequencies of the bulk recombinant populations derived from these two methods were largely similar. These results demonstrate that RNAi is a viable alternative for rapidly generating recombinant populations, allowing greater flexibility in experimental design.
是一个易于处理的模型系统，能够识别表型变异背后的遗传决定因素。多年来，已经开发出了新方法来降低该模型系统中基因定位的成本并加快其进程。X-QTL方法利用等位基因创建用于选择和测序实验的强制异交重组群体。在这里，我们通过比较N2和XZ1516菌株之间使用或RNAi敲低以促进异交的杂交，来测试等位基因对于X-QTL方法是否必不可少。源自这两种方法的大量重组群体的全基因组等位基因频率在很大程度上相似。这些结果表明，RNAi是快速产生重组群体的可行替代方法，在实验设计中具有更大的灵活性。
