Pieper Jason B, VanDusseldorp Payton, Kreuder Amanda J, Berger Darren J
Department of Veterinary Clinical Sciences, Iowa State University, Ames, Iowa, USA.
Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
Vet Dermatol. 2025 Oct;36(5):621-629. doi: 10.1111/vde.13355. Epub 2025 May 9.
Canine superficial bacterial folliculitis (SBF) is a common recurrent condition with a growing requirement for culture and susceptibility as a consequence of increased antimicrobial resistance and antimicrobial stewardship. Different opinions persist about the ideal technique for culture sample acquisition, which varies with lesion characteristics.
To determine if a difference exists in the recovered quantity of bacteria acquired when sampling nonexudative clinical lesions of SBF (crusts and epidermal collarettes) between a dry culture swab and a saline-moistened culture swab.
Nine dogs with crusts or epidermal collarettes and cytological findings consistent with SBF were recruited.
Each clinical lesion was divided into two halves. One half of the lesion was sampled with a dry cotton sterile swab, while the other half was sampled with a sterile swab saturated with sterile saline. Each lesion was sampled with the culture swab by rolling it over the corresponding half four times. Aerobic quantitative cultures were then performed to determine the number of Staphylococcus-type colonies present.
A total of 19 lesions (13 crusts and six epidermal collarettes) were evaluated. Dry culture swab identified a mean staphylococcal bacterial count of 3.83 Log colony-forming units (cfu)/mL (standard deviation [SD] = 0.70). The saline-soaked culture swab revealed a median bacterial count of 4.41 Log cfu/mL (SD = 0.77). There was a statistically significant difference between the sample collection methods (p = 0.002).
Sterile saline-soaked swabs are more likely than dry swabs to result in increased recovery of bacteria from nonexudative lesions.
犬浅表细菌性毛囊炎（SBF）是一种常见的复发性疾病，由于抗菌药物耐药性增加和抗菌药物管理的需要，对培养和药敏试验的需求也在不断增加。对于理想的培养样本采集技术存在不同意见，这因病变特征而异。
确定在对SBF的非渗出性临床病变（结痂和表皮项圈）进行采样时，干燥培养拭子和生理盐水浸湿的培养拭子所获取的细菌回收量是否存在差异。
招募了9只患有结痂或表皮项圈且细胞学检查结果与SBF一致的犬。
每个临床病变分为两半。病变的一半用干燥的无菌棉拭子采样，另一半用饱和无菌生理盐水的无菌拭子采样。每个病变用培养拭子在相应的一半上滚动四次进行采样。然后进行需氧定量培养以确定存在的葡萄球菌型菌落数量。
共评估了19个病变（13个结痂和6个表皮项圈）。干燥培养拭子鉴定出葡萄球菌平均细菌计数为3.83 Log菌落形成单位（cfu）/mL（标准差[SD]=0.70）。生理盐水浸湿的培养拭子显示细菌计数中位数为4.41 Log cfu/mL（SD=0.77）。样本采集方法之间存在统计学显著差异（p=0.002）。
无菌生理盐水浸湿的拭子比干燥拭子更有可能从非渗出性病变中提高细菌回收率。
