Huang Chun-Che, Chen Han-Wei, Hsieh Jo-Wei Allison, Lin Yen-Chun, Li Yi-Pei, Chen Chunxian, Song Yen-Fang, Yin Gung-Chian, Mai Te-Lun, Lin Ying-Chung Jimmy, Tu Yuan-Kai
Crop Genetic Resources and Biotechnology Division, Taiwan Agricultural Research Institute, Taichung, 413008, Taiwan.
Department of Life Science, National Taiwan University, Taipei, 10617, Taiwan.
BMC Plant Biol. 2025 May 10;25(1):620. doi: 10.1186/s12870-025-06622-7.
Trichomes in Prunus persica (L.) Batsch are crucial specialized structures that play a protective role against both biotic and abiotic stresses. The fruits with and without trichomes are respectively named as peach and nectarine. At the genetic level, the formation of trichome in peach is controlled by a single gene, PpMYB25, at the G locus. Peach (GG or Gg) is dominant to nectarine (gg), but such regulatory role was reported in a small-scale accession. In this study, we performed large-scale genotype and phenotype screening on 295 accessions. Almost all accessions supported the casual relationship between trichome formation and PpMYB25. However, a peach to nectarine mutant, named Maravilha Nectarine Mutant (MN), was discovered to possess a putative functional PpMYB25 gene sequence (Gg) but revealed nectarine phenotype. Comparative transcriptomic analyses revealed that PpMYB25 transcript was absent in MN. Correlation analyses also demonstrated that the PpMYB25-mediated regulatory network was abolished in MN. In summary, our results demonstrated an alternative mechanism beyond genetic regulation on trichome formation.
桃（Prunus persica (L.) Batsch）中的表皮毛是关键的特化结构，对生物和非生物胁迫均起到保护作用。有表皮毛和无表皮毛的果实分别被称为毛桃和油桃。在基因层面，桃中表皮毛的形成由位于G位点的单个基因PpMYB25控制。桃（GG或Gg）对油桃（gg）呈显性，但这种调控作用仅在小规模种质中被报道。在本研究中，我们对295份种质进行了大规模的基因型和表型筛选。几乎所有种质都支持表皮毛形成与PpMYB25之间的因果关系。然而，发现一个从桃到油桃的突变体，命名为“Maravilha油桃突变体”（MN），其具有推定的功能性PpMYB25基因序列（Gg），但表现出油桃表型。比较转录组分析表明MN中不存在PpMYB25转录本。相关性分析还表明MN中PpMYB25介导的调控网络被废除。总之，我们的结果证明了表皮毛形成的遗传调控之外的另一种机制。
