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糖尿病患者富血小板纤维蛋白的新型超微结构形态学特征

A Novel Ultrastructural Morphological Characterization of Platelet-Rich Fibrin among Diabetics.

作者信息

Rudresh Vinaya, Aghanashini Suchetha, Srirangarajan S, Srikumar P, Rao Ravi J, Upasana B

机构信息

Department of Periodontics, Krishnadevaraya College of Dental Sciences, Begaluru, Karnataka, India.

Department of Periodontics, R.V. Dental College, Begaluru, Karnataka, India.

出版信息

J Microsc Ultrastruct. 2023 Mar 22;13(1):25-31. doi: 10.4103/jmau.jmau_103_22. eCollection 2025 Jan-Mar.

DOI:10.4103/jmau.jmau_103_22
PMID:40351745
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12063922/
Abstract

BACKGROUND

The basic biology of the Platelet Rich Fibrin lies in the fact that they have platelets with enclosed granules in them and fibrin with the ability to release growth factors.

AIMS AND OBJECTIVE

The platelet rich fibrin electron microscopic structure has been characterized in the present cross-sectional novel study. This unique biologic material being vastly used since four decades is not microscopically analyzed amongst type II diabetes mellitus subjects, using light microscopy and transmission electron microscopy. The present novel work employs the transmission electron microscope use to reveal the characteristics of cells in order to correlate the growth factor release.

MATERIALS AND METHODS

Venous blood samples drawn were subjected to analysis of HbA1c, CBC, platelet indices, and PRF membrane preparation. Platelet rich fibrin membranes were prepared from healthy, well-controlled, and poorly-controlled diabetic individuals following the protocols for Advanced-Platelet Rich Fibrin+ (1,300 rpm, 8 min) and subjected to assessment of morphological analysis using a light microscope, transmission electron microscopy and growth factor release.

RESULTS

A denser network of fibrin with highest growth factor release was seen in the present study.

CONCLUSION

Hence, with the highest growth factors release and a denser network of fibrin, this novel study finds promising biomaterial in diabetics.

摘要

背景

富含血小板纤维蛋白的基本生物学特性在于其含有包裹着颗粒的血小板以及具有释放生长因子能力的纤维蛋白。

目的

在当前这项横断面新颖研究中，对富含血小板纤维蛋白的电子显微镜结构进行了表征。这种独特的生物材料自四十年来被广泛使用，但在II型糖尿病患者中，尚未使用光学显微镜和透射电子显微镜进行微观分析。本项新颖研究采用透射电子显微镜来揭示细胞特征，以便关联生长因子的释放。

材料与方法

采集的静脉血样本进行糖化血红蛋白、全血细胞计数、血小板指标分析以及富血小板纤维蛋白膜制备。按照高级富血小板纤维蛋白+方案（1300转/分钟，8分钟），从健康、血糖控制良好和控制不佳的糖尿病个体中制备富血小板纤维蛋白膜，并使用光学显微镜、透射电子显微镜和生长因子释放评估进行形态学分析。

结果

在本研究中观察到纤维蛋白网络更密集，生长因子释放量最高。

结论

因此，鉴于生长因子释放量最高且纤维蛋白网络更密集，本项新颖研究在糖尿病患者中发现了有前景的生物材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/adcf7eee0e69/JMAU-13-25-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/269a050b7661/JMAU-13-25-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/ea085238e721/JMAU-13-25-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/52d40a34e268/JMAU-13-25-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/b016f938ad98/JMAU-13-25-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/370dceb2ae90/JMAU-13-25-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/a6f1be03ea51/JMAU-13-25-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/adcf7eee0e69/JMAU-13-25-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/269a050b7661/JMAU-13-25-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/ea085238e721/JMAU-13-25-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/52d40a34e268/JMAU-13-25-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/b016f938ad98/JMAU-13-25-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/370dceb2ae90/JMAU-13-25-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/a6f1be03ea51/JMAU-13-25-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/12063922/adcf7eee0e69/JMAU-13-25-g007.jpg

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The Effect of Diabetes Mellitus on IGF Axis and Stem Cell Mediated Regeneration of the Periodontium.糖尿病对胰岛素样生长因子轴及干细胞介导的牙周组织再生的影响。
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