Bhowmik Amrita, Rokeya Begum, Kabir Yearul
Department of Applied Laboratory Sciences, Bangladesh University of Health Sciences, Dhaka, Bangladesh.
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh.
Int J Endocrinol. 2025 May 2;2025:5834412. doi: 10.1155/ije/5834412. eCollection 2025.
As a polygenic disorder, Type 2 diabetes mellitus is a prevalent disease developed by many multigenetic factors, of which multiple genes located on different chromosomes contribute to its susceptibility. The gene is involved in the Kir6.2 proteins that help release insulin from the potassium channels in pancreatic beta cells. Many studies have found that polymorphism is significantly associated with the incidence of T2DM. Therefore, this study was carried out to investigate the association between gene polymorphism and T2DM in the Bangladeshi population. In a case-control study ( = 697), 326 nondiabetic controls and 371 diabetic subjects (diagnosed based on American Diabetes Association criteria) were recruited for this study. The serum fasting glucose, lipid profiles, creatinine, alanine aminotransferase (ALT), HbA, and serum insulin level were measured by standard methods. HOMA-B%, HOMA-S%, and HOMA-IR were calculated using HOMA-SIGMA software Version 2.2. A standard formula calculated QUICKI and Secretory-HOMA. The chemical method was used for DNA extraction from whole blood samples. The PCR-RFLP method was used to detect polymorphisms by restriction enzyme (BanII) digestion. As appropriate, data were analyzed using an independent -test, chi-square, or Fisher exact test. < 0.05 was considered significant. The frequency of the risk allele K was significantly higher in the T2DM group than control ( ≤ 0.01). The frequency of the KK genotype was higher among the T2DM group (3.77% vs. 1.84%, < 0.05), and the frequency of the EK genotype was significantly higher among the T2DM than the control group (42.86% vs. 27.91%, < 0.001). The EE genotype was significantly associated with T2DM in the dominant model EE + EK with an OR of 2.06 (95% CI 1.51-2.82, ≤ 0.001). This study showed that rs5219 polymorphism of the gene is a significant risk factor for Type 2 diabetes mellitus in the Bangladeshi population.
作为一种多基因疾病，2型糖尿病是由多种多基因因素引发的常见疾病，位于不同染色体上的多个基因共同导致其易感性。该基因参与了Kir6.2蛋白的合成，这些蛋白有助于胰腺β细胞钾通道释放胰岛素。许多研究发现，[基因名称]多态性与2型糖尿病的发病率显著相关。因此，本研究旨在调查孟加拉人群中[基因名称]基因多态性与2型糖尿病之间的关联。在一项病例对照研究（n = 697）中，招募了326名非糖尿病对照者和371名糖尿病患者（根据美国糖尿病协会标准诊断）参与本研究。采用标准方法测量空腹血糖、血脂、肌酐、丙氨酸转氨酶（ALT）、糖化血红蛋白（HbA）和血清胰岛素水平。使用HOMA - SIGMA软件2.2版计算HOMA - B%、HOMA - S%和HOMA - IR。采用标准公式计算QUICKI和分泌型HOMA。采用化学方法从全血样本中提取DNA。采用PCR - RFLP方法通过限制性内切酶（BanII）消化检测[基因名称]多态性。根据情况，使用独立样本t检验、卡方检验或Fisher精确检验分析数据。P < 0.05被认为具有统计学意义。2型糖尿病组中风险等位基因K的频率显著高于对照组（P ≤ 0.01）。2型糖尿病组中KK基因型的频率更高（3.77%对1.84%，P < 0.05），2型糖尿病组中EK基因型的频率显著高于对照组（42.86%对27.91%，P < 0.001）。在显性模型EE + EK中，EE基因型与2型糖尿病显著相关，OR为2.06（95%CI 1.51 - 2.82，P ≤ 0.001）。本研究表明，[基因名称]基因的rs5219多态性是孟加拉人群中2型糖尿病的一个重要危险因素。
