Mirzaei Akram, Mashhadi Rahil, Aghsaeifard Ziba, Izadi Mehrnaz, Dougaheh Seyedeh Negin Hashemi, Omid Reza, Guitynavard Fateme, Nikoofar Parsa, Aghamir Seyed Mohammad Kazem
Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Urology, Thunder Bay Regional Health Research Institute, Thunder Bay, Ontario, Canada.
J Cell Mol Med. 2025 May;29(9):e70569. doi: 10.1111/jcmm.70569.
Considering the different behaviour of cells in response to diseases in different conditions and sex hormone-dependent cancers, we addressed the possible effect of the sex of the source of these cells from adipose tissue on prostate cancer cells. In this in vitro study, we evaluated the effects of male and female MSC Conditioned Media (MCM, FCM) on prostate cancer cells. The assessment included Hoechst dye staining, a scratch-wound assay, a colony formation assay, and a flow cytometric analysis of apoptosis and the cell cycle. We also performed real-time PCR to examine various genes, including apoptosis-related genes, epithelial-mesenchymal transition (EMT) genes, angiogenesis-related genes, and cell growth and survival biomarkers. Our results indicated that the IC values were 50% and 75% media in MCM and FCM in each of the three prostate cancer cell lines, respectively. An evaluation of gene expression revealed that in all three prostate cancer cell lines, treatment with MCM was more effective than FCM in reducing the expression of N-Cadherin and Vimentin, EGFR and BCL2 genes (p < 0.001). Furthermore, the MCM significantly increased the expression of BAX and E-Cadherin genes (p < 0.001) in the PC3 cell line. MCM proved to be more effective than FCM in reducing the expression of the epithelial-mesenchymal transition pathway, EGFR gene, and Apoptosis Regulator (BCL2) in the PC3 cell line. Due to its potential in regenerative medicine and cell therapy, this approach may serve as an effective treatment option for advanced prostate cancer.
考虑到细胞在不同条件下对疾病的不同反应以及性激素依赖性癌症，我们研究了来自脂肪组织的这些细胞的来源性别对前列腺癌细胞的可能影响。在这项体外研究中，我们评估了雄性和雌性间充质干细胞条件培养基（MCM、FCM）对前列腺癌细胞的影响。评估包括Hoechst染料染色、划痕试验、集落形成试验以及细胞凋亡和细胞周期的流式细胞术分析。我们还进行了实时PCR以检测各种基因，包括凋亡相关基因、上皮-间质转化（EMT）基因、血管生成相关基因以及细胞生长和存活生物标志物。我们的结果表明，在三种前列腺癌细胞系中，MCM和FCM的IC值分别为50%和75%培养基。基因表达评估显示，在所有三种前列腺癌细胞系中，用MCM处理在降低N-钙黏蛋白、波形蛋白、表皮生长因子受体（EGFR）和B细胞淋巴瘤-2（BCL2）基因的表达方面比FCM更有效（p<0.001）。此外，MCM显著增加了PC3细胞系中BAX和E-钙黏蛋白基因的表达（p<0.001）。在PC3细胞系中，MCM在降低上皮-间质转化途径、EGFR基因和凋亡调节因子（BCL2）的表达方面比FCM更有效。由于其在再生医学和细胞治疗中的潜力，这种方法可能成为晚期前列腺癌的有效治疗选择。
