Saied Norhan, Yaseen Rabaa, Mostafa Amal Abdelmonem, Barghout Marwa El-Desouky, Ahmed Rasha Hussein, Amin Basma Hamdy, Ali Mohamed Abdelalim
Agricultural Microbiology Department, Faculty of Agriculture, Cairo University, Cairo, Egypt.
Soil Fertility and Microbiology Department, Water Resources and Desert Soils Division, Desert Research Center, Cairo 11753, Egypt.
Recent Adv Antiinfect Drug Discov. 2025;20(2):139-156. doi: 10.2174/0127724344320368241021072156.
Infestation in greenhouse cucumber with the two-spotted spider mite ( Koch) commonly causes severe damage to crop quality and quantity and increases crop production costs.
This study was conducted to investigate the efficacy of high-abamectinproducing isolates of against -infested cucumber and to assess their impact on biochemical stress markers in these vegetables.
In this study, 72 non-antagonistic were isolated from rhizospheric soil samples collected from eight different locations in Egypt and screened for their ability to produce the secondary metabolite, abamectin.
The screening process identified two potent abamectin-producing isolates, EW8 and T2, which produced 42.7 and 29.6 μg/L abamectin, respectively, as confirmed by LC-MS/MS analysis. According to DNA sequence analysis of the 16S rRNA gene, these two isolates belong to the species . The acaricidal activity of either culture suspensions of strains WE8 and T2, or their extracts containing abamectin, against the mobile stages and egg hatchability of was evaluated in the laboratory and the greenhouse. Data on the mortality among the examined female mites and the reduction in their number of eggs point out a potential acaricidal activity of the examined strains of and their extracts containing abamectin against . Furthermore, the extracts containing abamectin from these two strains induced oxidative stress in the infested cucumber plants by , as indicated by increased levels of malondialdehyde (MDA). However, the levels of MDA in -infested cucumber plants varied depending on the strain and the specific abamectin crude extract used.
strains T2 or WE8, or their crude extract could be applied in greenhouse cucumber plantations to combat red mite infestation.
温室黄瓜受二斑叶螨（科赫）侵害通常会严重损害作物的质量和产量，并增加作物生产成本。
本研究旨在调查高产阿维菌素的菌株对受螨侵害黄瓜的防治效果，并评估其对这些蔬菜中生化应激标志物的影响。
本研究从埃及八个不同地点采集的根际土壤样本中分离出72株非拮抗菌株，并筛选它们产生次生代谢产物阿维菌素的能力。
筛选过程中鉴定出两株高效产阿维菌素的菌株EW8和T2，经液相色谱-串联质谱（LC-MS/MS）分析确认，它们分别产生42.7和29.6μg/L的阿维菌素。根据16S rRNA基因的DNA序列分析，这两株菌株属于该物种。在实验室和温室中评估了菌株WE8和T2的培养悬浮液或其含阿维菌素的提取物对二斑叶螨活动阶段和卵孵化率的杀螨活性。关于所检查雌螨死亡率及其产卵数量减少的数据表明，所检查的菌株及其含阿维菌素的提取物对二斑叶螨具有潜在的杀螨活性。此外，这两株菌株含阿维菌素的提取物在受螨侵害的黄瓜植株中诱导了氧化应激，这表现为丙二醛（MDA）水平升高。然而，受螨侵害黄瓜植株中MDA的水平因菌株和所用的特定阿维菌素粗提物而异。
菌株T2或WE8，或其粗提物可应用于温室黄瓜种植园中防治红蜘蛛螨害。
