Sun Dandan, Zhang Haijin, Wu Yanting, Zhou Jiawei, Ai Ling
Jiaxing Women and Children Hospital Affiliated to Wenzhou Medical University, Jiaxing, 314051, China.
Women and Children Hospital Affiliated to Jiaxing University, Jiaxing, 314051, China.
BMC Pregnancy Childbirth. 2025 May 13;25(1):567. doi: 10.1186/s12884-025-07663-4.
To investigate the expression of mitofusin 2 (Mfn2) in the placenta and peripheral blood of patients with early-onset preeclampsia (eoPE) and late-onset preeclampsia (loPE), and to evaluate its possibility as a diagnostic and therapeutic target for preeclampsia.
A total of 68 pregnant women with preeclampsia in Jiaxing Maternal and Child Health Hospital from June 2022 to June 2024 were selected, including 32 patients with eoPE and 36 patients with loPE, and 68 term pregnant women as negative controls. Real-time fluorescence reverse transcription (RT-qPCR) was used to determine the expression level of Mfn2 mRNA in placenta and peripheral blood, and the expression of Mfn2 was analyzed; enzyme-linked immunosorbent assay (ELISA) was used to determine the level of Mfn2 in peripheral blood of patients, and the outcomes of pregnant women (placental weight, neonatal birth weight, 1 min Apgar) were recorded. The correlation between the level of Mfn2 in peripheral blood and the severity of preeclampsia and pregnancy outcomes was analyzed.
The expression of Mfn2 mRNA in the placenta tissue of the eoPE group was significantly lower than that of the term pregnancy group and the loPE group (P < 0.001), while the expression of Mfn2 in the placenta tissue of the loPE group was only lower than that of the term pregnancy group, with no significant difference(P > 0.05). The expression of Mfn2 mRNA in the peripheral blood of eoPE and loPE was significantly higher than that of term pregnancy group (all P < 0.001). In the peripheral blood, the levels of Mfn2 in eoPE group and loPE group were significantly higher than that of the term pregnancy group (all P < 0.001). The Pearson correlation analysis showed that the expression level of Mfn2 protein in peripheral blood of patients are positively correlated with blood pressure and urinary protein, and negatively correlated with neonatal birth weight and 1 min Apgar score.
In early-onset placenta, the expression of Mfn2 is significantly lower than that in full-term pregnancy, while in late-onset eclampsia, it is not, indicating that the abnormal expression of Mnf2 in placenta is related to the occurrence period of preeclampsia and the progression of the disease; Mfn2 in peripheral blood can be used as a biological marker to evaluate the occurrence and progression of preeclampsia.
探讨早发型子痫前期（eoPE）和晚发型子痫前期（loPE）患者胎盘组织及外周血中丝裂原活化蛋白激酶2（Mfn2）的表达情况，并评估其作为子痫前期诊断和治疗靶点的可能性。
选取2022年6月至2024年6月在嘉兴市妇幼保健院就诊的68例子痫前期孕妇，其中早发型子痫前期患者32例，晚发型子痫前期患者36例，选取68例足月孕妇作为阴性对照。采用实时荧光逆转录（RT-qPCR）法检测胎盘组织及外周血中Mfn2 mRNA的表达水平，并分析Mfn2的表达情况；采用酶联免疫吸附测定（ELISA）法检测患者外周血中Mfn2水平，并记录孕妇结局（胎盘重量、新生儿出生体重、1分钟阿氏评分）。分析外周血中Mfn2水平与子痫前期严重程度及妊娠结局的相关性。
早发型子痫前期组胎盘组织中Mfn2 mRNA的表达水平显著低于足月妊娠组和晚发型子痫前期组（P＜0.001），而晚发型子痫前期组胎盘组织中Mfn2的表达仅低于足月妊娠组，差异无统计学意义（P＞0.05）。早发型子痫前期组和晚发型子痫前期组外周血中Mfn2 mRNA的表达水平显著高于足月妊娠组（均P＜0.001）。在外周血中，早发型子痫前期组和晚发型子痫前期组Mfn2水平显著高于足月妊娠组（均P＜0.001）。Pearson相关性分析显示，患者外周血中Mfn2蛋白表达水平与血压及尿蛋白呈正相关，与新生儿出生体重及1分钟阿氏评分呈负相关。
早发型子痫前期胎盘组织中Mfn2的表达显著低于足月妊娠，而晚发型子痫前期则不然，提示胎盘组织中Mnf2的异常表达与子痫前期的发病时期及病情进展有关；外周血中的Mfn2可作为评估子痫前期发生及进展的生物学标志物。
