Effective conservation of large mammals depends on how people perceive them. Grey wolves have a widespread distribution globally, and their recent recolonization of human-dominated landscapes offers an excellent opportunity to understand the heterogeneity in their perception across continents. Our analysis included all quantitative studies (118 articles) conducted in 35 countries through a systematic review process, published globally between 1980-2023 and indexed in Web of Science and Google Scholar. Fifty-four percent of the studies reported a negative perception toward wolves. Most studies conducted in Asia reported a negative perception, while 56% of studies conducted in Europe and 48% in North America reported a positive perception. Fifty-four percent of studies from Western Europe and forty percent of studies from Slavic Russian cultural regions reported positive perceptions. Respondents from low-income countries elicited the most negative perceptions. We identified the predominant religion and economic status of a country as dominant factors determining perception. Studies conducted in countries with Hinduism as the predominant religion reported a negative perception toward wolves. We recommend that future studies on human-wolf interactions must prioritize regions within central Europe, parts of Asia, and Russia. A global human-wolf coexistence strategy should consider the social factors driving attitude toward the species.