Marchi Pedro Henrique, Príncipe Leonardo de Andrade, Trindade Felipe Sesti, Santos Luana Dias Dos, Finardi Gabriela Luiza Fagundes, Fernandes Eduarda Lorena, Putarov Thaila Cristina, Ribeiro Gabriel Henrique, Colnago Luiz Alberto, Balieiro Júlio Cesar de Carvalho, Vendramini Thiago Henrique Annibale
Pet Nutrology Research Center (CEPEN Pet), Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Pirassununga 13635-900, Brazil.
Biorigin (Açucareira Quatá S.A.), Lençois Paulistas 18680-900, Brazil.
Animals (Basel). 2025 Apr 24;15(9):1211. doi: 10.3390/ani15091211.
Metabolomics has proven to be an effective tool for elucidating mechanisms and assessing the effectiveness of dietary interventions in canine and feline nutrition. In this context, the present study aimed to perform a metabolomic analysis of the serum of dogs supplemented with increasing levels of beta-1,3/1,6-glucans to generate evidence and gain a deeper understanding of the metabolic responses associated with this supplementation. Eight dogs were evenly assigned to two balanced 4 × 4 Latin squares. Four diets were tested, differing only in beta-glucan content (0.0%, 0.07%, 0.14%, and 0.28%), and the dogs were fed according to their individual maintenance energy requirements. Each experimental period lasted 35 days. On day 35, 5 mL of blood was collected via jugular venipuncture to obtain serum for metabolomic analysis. Nuclear magnetic resonance analysis identified 12 key serum metabolites. Hierarchical heat map analysis revealed differences in metabolite intensity between treatments ( < 0.05). Additionally, the most relevant metabolic pathways were phenylalanine, tyrosine, and tryptophan metabolism; alanine, aspartate, and glutamate metabolism; and glyoxylate and dicarboxylate metabolism. This study demonstrated that increasing levels of purified beta-1,3/1,6-glucans from modulated key metabolic pathways in dogs, particularly those related to amino acid, lipid and energy metabolisms, and gut microbiota. These findings provide insights into the mechanisms by which beta-glucans influence canine health.
代谢组学已被证明是一种有效的工具，可用于阐明犬猫营养中饮食干预的机制并评估其有效性。在此背景下，本研究旨在对补充不同水平β-1,3/1,6-葡聚糖的犬血清进行代谢组学分析，以获取证据并更深入地了解与这种补充相关的代谢反应。八只狗被均匀分配到两个平衡的4×4拉丁方设计中。测试了四种饮食，仅β-葡聚糖含量不同（0.0%、0.07%、0.14%和0.28%），并根据每只狗的个体维持能量需求进行喂养。每个实验期持续35天。在第35天，通过颈静脉穿刺采集5毫升血液以获得用于代谢组学分析的血清。核磁共振分析确定了12种关键血清代谢物。层次热图分析揭示了不同处理之间代谢物强度的差异（<0.05）。此外，最相关的代谢途径是苯丙氨酸、酪氨酸和色氨酸代谢；丙氨酸、天冬氨酸和谷氨酸代谢；以及乙醛酸和二羧酸代谢。本研究表明，增加纯化的β-1,3/1,6-葡聚糖水平可调节犬的关键代谢途径，特别是那些与氨基酸、脂质和能量代谢以及肠道微生物群相关的途径。这些发现为β-葡聚糖影响犬健康的机制提供了见解。
