Blyakhman Felix, Fadeyev Fedor, Safronov Alexander, Terziyan Tatiana, Burban Ekaterina, Shklyar Tatyana, Kurlyandskaya Galina
Department of Biomedical Physics, Ural State Medical University, Ekaterinburg 620028, Russia.
Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620002, Russia.
Materials (Basel). 2025 May 6;18(9):2142. doi: 10.3390/ma18092142.
Administration of monocyte-derived dendritic cells (moDCs) sensitized by cancer-associated antigens to the patient is applied to boost the T-cell mediated anti-tumor immune response. Loading moDCs with magnetic nanoparticles (MNPs) and controlling their migration to lymph nodes by an external magnetic field is a way to improve the effectiveness of immunotherapy. In this study, spherical MNPs of maghemite iron oxide with a diameter of about 14 nm were synthesized by laser target evaporation method (LTE) and examined in the context of their prospective use for the needs of moDCs immunotherapy. Characterization of the physicochemical properties of MNPs and their stabilization in physiological media, as well as the magnetic properties of MNPs in the suspensions were considered in detail. The cytotoxic effect of MNPs in growth medium on the human moDCs and MNPs uptake by the cells were also estimated. We show that up-taken MNPs and MNPs in growth medium demonstrated cytotoxic effect only at high concentrations. At the same time, at low concentrations MNPs up-taken by moDCs increased their viability causing the stimulation effect. The evaluation of the quantity of MNPs, up-taken by cells, is possible by magnetometry even for the smallest γ-FeO concentrations.
将由癌症相关抗原致敏的单核细胞衍生树突状细胞（moDCs）给予患者，以增强T细胞介导的抗肿瘤免疫反应。用磁性纳米颗粒（MNPs）负载moDCs并通过外部磁场控制其向淋巴结的迁移是提高免疫治疗效果的一种方法。在本研究中，采用激光靶蒸发法（LTE）合成了直径约为14nm的磁赤铁矿球形MNPs，并对其在moDCs免疫治疗中的潜在应用进行了研究。详细考虑了MNPs的物理化学性质及其在生理介质中的稳定性，以及悬浮液中MNPs的磁性。还评估了生长培养基中MNPs对人moDCs的细胞毒性作用以及细胞对MNPs的摄取情况。我们发现，摄取的MNPs和生长培养基中的MNPs仅在高浓度时表现出细胞毒性作用。同时，在低浓度下，moDCs摄取的MNPs可提高其活力，产生刺激作用。即使对于最小的γ-FeO浓度，通过磁力测定法也可以评估细胞摄取的MNPs数量。
