Schroers Maike, Zablotski Yuri, Meyer-Lindenberg Andrea
Clinic of Small Animal Surgery and Reproduction, Veterinary Faculty, Ludwig-Maximilians-Universität München, Munich, Germany.
J Vet Diagn Invest. 2025 May 14:10406387251327413. doi: 10.1177/10406387251327413.
We determined saliva cortisol concentrations (SCCs) in samples from 36 dogs using a chemiluminescence immunoassay (CLIA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) before and after a 10-min veterinary visit. The median cortisol concentration of the 77 samples analyzed with CLIA was 1.34 nmol/L, and the concentration of samples analyzed with LC-MS/MS was 1.30 nmol/L. With an intercept (a) of -0.06 (95% CI: -0.16 to 0.02) and a slope (b) of 0.97 (95% CI: 0.90-0.97) the Passing-Bablok regression revealed that there were no significant systematic and proportional differences between the 2 methods. In the Bland-Altman analysis, the bias was 0.08 nmol/L (95% CI: -0.38 to 0.54 nmol/L) between the 2 methods. Comparing the hormone levels in the saliva of 36 dogs before and after a veterinary visit, the CLIA method had a mean difference of 0.03 nmol/L (95% CI: -1.72 to 4.75 nmol/L; = 0.35) and the LC-MS/MS method a mean difference of 0.12 nmol/L (95% CI 95%: -2.43 to 4.40 nmol/L; = 0.62). Comparison of cortisol changes before ( = 0.86) and after ( 0.79) the veterinary visit revealed no differences in using either the CLIA or LC-MS/MS methods. There was also no difference in the assessment of hormone increase or decrease between CLIA ( = 0.72) and LC-MS/MS ( = 0.17). Overall, the measurement of SCC in dogs by either CLIA or LC-MS/MS provided comparable results.
我们在36只犬的样本中，于10分钟的兽医诊疗前后，使用化学发光免疫分析法（CLIA）和液相色谱 - 串联质谱法（LC-MS/MS）测定唾液皮质醇浓度（SCC）。用CLIA分析的77个样本的皮质醇浓度中位数为1.34 nmol/L，用LC-MS/MS分析的样本浓度为1.30 nmol/L。通过Passing-Bablok回归分析，截距（a）为 -0.06（95%可信区间：-0.16至0.02），斜率（b）为0.97（95%可信区间：0.90 - 0.97），结果显示两种方法之间无显著的系统差异和比例差异。在Bland-Altman分析中，两种方法之间的偏差为0.08 nmol/L（95%可信区间：-0.38至0.54 nmol/L）。比较36只犬在兽医诊疗前后唾液中的激素水平，CLIA法的平均差异为0.03 nmol/L（95%可信区间：-1.72至4.75 nmol/L；P = 0.35），LC-MS/MS法的平均差异为0.12 nmol/L（95%可信区间：-2.43至4.40 nmol/L；P = 0.62）。比较兽医诊疗前（P = 0.86）和诊疗后（P = 0.79）皮质醇的变化，结果显示使用CLIA或LC-MS/MS方法均无差异。在评估激素升高或降低方面，CLIA（P = 0.72）和LC-MS/MS（P = 0.17）之间也无差异。总体而言，通过CLIA或LC-MS/MS测量犬的SCC可提供可比的结果。
