Li Liying, Liu Xinyue, Sun Huamao, Ma Hailin, Cheng Yuen Yee, Li Xiangqin, Jia Zhilin, Zhao Jiaquan, Song Kedong
State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Cancer Hospital of Dalian University of Technology, Dalian University of Technology, Dalian, China.
Mathematics Teaching and Research Group, The High School Attached to Dalian University of Technology, Dalian, China.
Biotechnol J. 2025 May;20(5):e70039. doi: 10.1002/biot.70039.
Bioreactor technology facilitates the gradual automation of cell expansion and the development of biofunctional synthetic alternatives. However, it is difficult to fully understand the flow field and force field environments formed in it by experimental means. Computational fluid dynamics (CFD) offers a robust framework for analyzing and understanding the impacts of fluid flow, material diffusion, and fluid shear stress (FSS) on in vitro cell and tissue regeneration dynamics. In this study, the FLUENT software is used to simulate and calculate the flow field environment of the rotary cell culture system (RCCS) and spinner flask (SF), including dynamic pressure, shear stress, and velocity distribution. Particles of two diameters for three-dimensional cell culture were randomly arranged in different radial/axial positions, and the FSS on the particles in RCCS and SF at different rotational speeds was also analyzed. It is expected to visualize the flow field distribution of the bioreactor and local hydrodynamic changes near the particles, and provide positive assistance for the dynamic culture/co-culture of different cells-microcarriers complex. The distribution of FSS on randomly arranged L and S particles was analyzed in detail to evaluate and screen the suitable operating conditions of these two bioreactors. Visually understanding the flow field distribution and local hydrodynamic changes within the bioreactor is expected to provide positive assistance for dynamic culture. The particles may periodically contact the fresh oxygenated medium during rotation with the fluid. Two fluid circulations in SF were generated in the upper/lower area of the blade, and a relatively static fluid circulation area was formed at the bottom with low velocity and pressure in the center, which was not conducive to material exchange. Rotary bioreactors may be more suitable than spinner flasks as a dynamic culture tool for some types of cells or other constructs.
生物反应器技术有助于细胞扩增的逐步自动化以及生物功能合成替代物的开发。然而，通过实验手段很难完全了解其中形成的流场和力场环境。计算流体动力学（CFD）为分析和理解流体流动、物质扩散以及流体剪切应力（FSS）对体外细胞和组织再生动力学的影响提供了一个强大的框架。在本研究中，使用FLUENT软件对旋转细胞培养系统（RCCS）和转瓶（SF）的流场环境进行模拟和计算，包括动压、剪切应力和速度分布。将用于三维细胞培养的两种直径的颗粒随机排列在不同的径向/轴向位置，并分析了RCCS和SF中不同转速下颗粒上的FSS。期望能够可视化生物反应器的流场分布以及颗粒附近的局部流体动力学变化，并为不同细胞 - 微载体复合物的动态培养/共培养提供积极的帮助。详细分析了随机排列的L和S颗粒上FSS的分布，以评估和筛选这两种生物反应器的合适操作条件。直观地了解生物反应器内的流场分布和局部流体动力学变化有望为动态培养提供积极的帮助。颗粒在随流体旋转过程中可能会周期性地接触新鲜的含氧培养基。在SF中，叶片的上部/下部区域产生了两个流体循环，在底部形成了一个相对静止的流体循环区域，其中心速度和压力较低，不利于物质交换。作为某些类型细胞或其他构建体的动态培养工具，旋转生物反应器可能比转瓶更合适。
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